CD138/Syndecan-1 Polyclonal antibody

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  WB analysis using 10593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10593-1-AP

  WB result of CD138/Syndecan-1 antibody (10593-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD138/Syndecan-1 transfected HeLa cells.

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  IHC staining of human tonsillitis using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

CD138/Syndecan-1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10593-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

CD138, SDC, SDC1, SYND1, syndecan

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 13 publications below
• IF: See 11 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

10593-1-AP targets CD138/Syndecan-1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0823

  Product name: Recombinant human SDC1,CD138 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC008765

  Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA

• Full Name: syndecan 1
• Calculated Molecular Weight: 32.5 kDa
• Observed Molecular Weight: 60-100 kDa, 130 kDa
• GenBank Accession Number: BC008765
• Gene Symbol: CD138
• Gene ID (NCBI): 6382
• RRID: AB_2182736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18827
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Cell; Tumor-reactive antibodies evolve from non-binding and autoreactive precursors.; Authors - Roei D Mazor
human	WB,IP	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	IHC	Leukemia; miR-21 antagonism abrogates Th17 tumor promoting functions in multiple myeloma.; Authors - Marco Rossi
mouse	IF	Theranostics; Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.; Authors - Xin Chen
		Mol Neurodegener; Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease model.; Authors - Yi-Ling Liu
human	WB	Cell Mol Life Sci; The association of telomere maintenance and TERT expression with susceptibility to human papillomavirus infection in cervical epithelium.; Authors - Li-Na Wang