SEC16A Polyclonal antibody

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  WB analysis of MCF-7 using 20025-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20025-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20025-1-AP

  IP result of anti-SEC16A (IP:20025-1-AP, 4ug; Detection:20025-1-AP 1:2000) with HeLa cells lysate 840 ug.

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  IHC staining of mouse pancreas using 20025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 20025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 20025-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SEC16A antibody (20025-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 20025-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEC16A (20025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

SEC16A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 20025-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

SEC16L, SEC16 homolog A, SEC16, RP11 413M3.10, p250

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

20025-1-AP targets SEC16A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SEC16 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 234 kDa
• Observed Molecular Weight: 250-300 kDa
• GenBank Accession Number: NM_014866
• Gene Symbol: SEC16A
• Gene ID (NCBI): 9919
• RRID: AB_2878635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15027
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nat Chem Biol; Functional dissection of the retrograde Shiga toxin trafficking inhibitor Retro-2.; Authors - Alison Forrester ; KD Validated
rat	WB	Cell Death Differ; C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport; Authors - Yachen Wu
mouse	IF	Mol Neurodegener; Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Panpan Wang