SEC31A Polyclonal antibody

• Featured Product
• KD/KO Validated

SEC31A Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (3)

Cat no : 17913-1-AP

Synonyms

ABP125, ABP130, HSPC275, HSPC334, KIAA0905, SEC31 like protein 1, SEC31 related protein A, SEC31A, SEC31L1, Web1 like protein

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Validation Data Gallery

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  WB analysis of HeLa using 17913-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17913-1-AP (SEC31A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17913-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17913-1-AP (SEC31A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17913-1-AP

  IP Result of anti-SEC31A (IP:17913-1-AP, 4ug; Detection:17913-1-AP 1:500) with HeLa cells lysate 1080ug.

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  IF Staining of HeLa using 17913-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC31A antibody (17913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 17913-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17913-1-AP (SEC31A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

17913-1-AP targets SEC31A in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC31A fusion protein Ag12314
• Full Name: SEC31 homolog A (S. cerevisiae)
• Calculated molecular weight: 1220 aa, 133 kDa
• Observed molecular weight: 133 kDa
• GenBank accession number: BC084583
• Gene symbol: SEC31A
• Gene ID (NCBI): 22872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

Publications

Species	Application	Title
human	IP,WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long ; KO Validated
mouse	IF	Cell; Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.; Authors - Moran Dvela-Levitt
human	IF	Dev Cell; SURF4-induced tubular ERGIC selectively expedites ER-to-Golgi transport.; Authors - Rui Yan
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
rat	WB	Cell Death Dis; ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons.; Authors - Tatiana Leiva-Rodríguez

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (09-01-2020)

This antibody labels a strong band around the expected molecular weight.

• Applications: Western Blot

FH

RICHA (Verified Customer) (09-29-2019)

Clean, excellent and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

SEC31A Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:17913-1-AP)

FH

Rui (Verified Customer) (09-28-2019)

Labels intracellular vesicles with clear contrast. Cell monolayer fixed by 0.3% glutaraldehyde and 3% paraformaldehyde. Permeabilization: 0.1% saponin and 3% BSA in PBS. Primary antibody overnight at 4C.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Cos-7

SEC31A Antibody Immunofluorescence, validation (1:300 dilution) in Cos-7 (Cat no:17913-1-AP)