SENP8 Polyclonal antibody, PBS Only

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  IHC staining of human colon cancer using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of rat pancreas using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 13479-1-AP (same clone as 13479-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13479-1-AP (SENP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13479-1-AP (same clone as 13479-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP8 antibody (13479-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13479-1-PBS in a different storage buffer formulation.

SENP8 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 13479-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

HsT17512, FKSG8, EC:3.4.22.-, Deneddylase-1, Deneddylase 1

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Product Information

13479-1-PBS targets SENP8 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SENP8 fusion protein Ag4287
• Full Name: SUMO/sentrin specific peptidase family member 8
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC031411
• Gene Symbol: SENP8
• Gene ID (NCBI): 123228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96LD8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SENP8, a human NEDD8-specific protease, also known as NEDP1. It is expected to be located in cytoplasm and nucleus, which is highly expressed in kidney and pancreas. The calculated molecular weight of SENP8 is 24 kDa. It is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53 (PMID: 15242646). A single-residue difference in the C-terminus of NEDD8 and ubiquitin contributes significantly to the ability of NEDP1 to discriminate between them. In vivo analysis indicates that NEDP1 mutants perturb deNEDDylation of the tumour suppressor p53 (PMID: 12759363).