SEPT6 Monoclonal antibody, PBS Only

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  WB analysis using 68173-1-Ig (same clone as 68173-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68173-1-Ig (SEPT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68173-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68173-1-Ig (same clone as 68173-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody (68173-1-Ig, Clone: 1B1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68173-1-PBS in a different storage buffer formulation.

SEPT6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68173-1-PBS

Clone No.1B1G1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0128, SEP2, SEPT2, SEPT6, septin 6

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Product Information

68173-1-PBS targets SEPT6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

• Tested Reactivity: Human, mouse, pig, rabbit, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32736

  Product name: Recombinant human SEPT6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 86-434 aa of BC036240

  Sequence: YDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE

• Full Name: septin 6
• Calculated Molecular Weight: 434 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036240
• Gene Symbol: SEPT6
• Gene ID (NCBI): 23157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14141
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.