Alpha 1 Antitrypsin Polyclonal antibody

Alpha 1 Antitrypsin Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 16382-1-AP

Synonyms

A1A, A-1-Antitrypsin, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT, α1-antitrypsin

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Validation Data Gallery

• 

  WB analysis using 16382-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16382-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16382-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16382-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 16382-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16382-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human urine using 16382-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16382-1-AP

  IP Result of anti-Alpha-1-Antitrypsin (IP:16382-1-AP, 4ug; Detection:16382-1-AP 1:1000) with mouse kidney tissue lysate 7200ug.

• 

  IHC staining of human liver cancer using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16382-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 16382-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Alpha-1-Antitrypsin antibody (16382-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: human saliva tissue, human kidney tissue, human urine sample, mouse liver tissue, mouse skin tissue, mouse kidney tissue, mouse lung tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue, human hepatocirrhosis tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

16382-1-AP targets Alpha 1 Antitrypsin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha 1 Antitrypsin fusion protein Ag9369
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
• Calculated molecular weight: 418 aa, 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC015642
• Gene symbol: SERPINA1
• Gene ID (NCBI): 5265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

Publications

Species	Application	Title
human	WB	Am J Physiol Renal Physiol; Free circulating active elastase, contributes to chronic inflammation in hemodialysis patients.; Authors - Eman Khatib-Massalha
human	WB	Dis Markers; Identification of differentially expressed serum proteins in infectious purpura fulminans.; Authors - Ting He
human	WB	Med Oncol; High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques.; Authors - Qin Xue X
human	WB	Transl Androl Urol; Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.; Authors - Xuechao Li
human	WB	Mol Reprod Dev; Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins.; Authors - Tian An
mouse	WB	PLoS One; Knockdown of Alpha-1 Antitrypsin with antisense oligonucleotide does not exacerbate smoke induced lung injury.; Authors - Kyle Stearns

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