Alpha 1 Antitrypsin Polyclonal antibody
Alpha 1 Antitrypsin Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 16382-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human saliva tissue, human kidney tissue, human urine sample, mouse liver tissue, mouse skin tissue, mouse kidney tissue, mouse lung tissue |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human liver cancer tissue, human hepatocirrhosis tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 14 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
Product Information
16382-1-AP targets Alpha 1 Antitrypsin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Alpha 1 Antitrypsin fusion protein Ag9369 |
Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 |
Calculated molecular weight | 418 aa, 47 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC015642 |
Gene symbol | SERPINA1 |
Gene ID (NCBI) | 5265 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.
Protocols
Product Specific Protocols | |
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WB protocol for Alpha 1 Antitrypsin antibody 16382-1-AP | Download protocol |
IHC protocol for Alpha 1 Antitrypsin antibody 16382-1-AP | Download protocol |
IP protocol for Alpha 1 Antitrypsin antibody 16382-1-AP | Download protocol |
FC protocol for Alpha 1 Antitrypsin antibody 16382-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Am J Physiol Renal Physiol Free circulating active elastase, contributes to chronic inflammation in hemodialysis patients. | ||
Dis Markers Identification of differentially expressed serum proteins in infectious purpura fulminans. | ||
Med Oncol High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques. | ||
Transl Androl Urol Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples. | ||
Mol Reprod Dev Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins. | ||
PLoS One Knockdown of Alpha-1 Antitrypsin with antisense oligonucleotide does not exacerbate smoke induced lung injury. |