Alpha 1 Antitrypsin Polyclonal antibody

Alpha 1 Antitrypsin Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 16382-1-AP

Synonyms

A1A, A-1-Antitrypsin, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT, α1-antitrypsin



Tested Applications

Positive WB detected inhuman saliva tissue, human kidney tissue, human urine sample, mouse liver tissue, mouse skin tissue, mouse kidney tissue, mouse lung tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16382-1-AP targets Alpha 1 Antitrypsin in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha 1 Antitrypsin fusion protein Ag9369
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Calculated molecular weight 418 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC015642
Gene symbol SERPINA1
Gene ID (NCBI) 5265
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

Protocols

Product Specific Protocols
WB protocol for Alpha 1 Antitrypsin antibody 16382-1-APDownload protocol
IHC protocol for Alpha 1 Antitrypsin antibody 16382-1-APDownload protocol
IP protocol for Alpha 1 Antitrypsin antibody 16382-1-APDownload protocol
FC protocol for Alpha 1 Antitrypsin antibody 16382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Physiol Renal Physiol

Free circulating active elastase, contributes to chronic inflammation in hemodialysis patients.

Authors - Eman Khatib-Massalha
humanWB

Dis Markers

Identification of differentially expressed serum proteins in infectious purpura fulminans.

Authors - Ting He
humanWB

Med Oncol

High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques.

Authors - Qin Xue X
humanWB

Mol Reprod Dev

Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins.

Authors - Tian An
humanWB,IHC

Onco Targets Ther

Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells.

Authors - Dong-Ming Wu
ratWB

J Proteomics

Proteomic analysis reveals the protective effects of emodin on severe acute pancreatitis induced lung injury by inhibiting neutrophil proteases activity.

Authors - Caiming Xu