Validation Data Gallery
|Positive WB detected in||human blood tissue, COLO 320 cells, human colon tissue|
|Positive IP detected in||COLO 320 cells|
|Positive IHC detected in||human tonsillitis tissue, human liver tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12192-1-AP targets Alpha Antichymotrypsin in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha Antichymotrypsin fusion protein Ag2830|
|Full Name||serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3|
|Calculated molecular weight||448 aa, 48 kDa|
|Observed molecular weight||44-70 kDa|
|GenBank accession number||BC010530|
|Gene ID (NCBI)||12|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.
|Product Specific Protocols|
|WB protocol for Alpha Antichymotrypsin antibody 12192-1-AP||Download protocol|
|IHC protocol for Alpha Antichymotrypsin antibody 12192-1-AP||Download protocol|
|IP protocol for Alpha Antichymotrypsin antibody 12192-1-AP||Download protocol|
|FC protocol for Alpha Antichymotrypsin antibody 12192-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Screening and identification of tissue-infiltrating immune cells and genes for patients with emphysema phenotype of COPD
Br J Cancer
Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.
Front Med (Lausanne)
An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.
Proteomics Clin Appl
Serum protein profiling in diffuse large B-cell lymphoma.
Sinensetin attenuates IL-1β-induced cartilage damage and ameliorates osteoarthritis by regulating SERPINA3
Acta Neuropathol Commun
Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortex