Alpha Antichymotrypsin Polyclonal antibody

Alpha Antichymotrypsin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 12192-1-AP

Synonyms

SERPINA3, Alpha-1-antichymotrypsin, Alpha 1 antichymotrypsin, ACT, AACT

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Validation Data Gallery

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  WB analysis of human plasma using 12192-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12192-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12192-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12192-1-AP

  IP result of anti-Alpha Antichymotrypsin (IP:12192-1-AP, 4ug; Detection:12192-1-AP 1:500) with COLO 320 cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12192-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HepG2 using 12192-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human blood tissue, COLO 320 cells, human colon tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human tonsillitis tissue, human liver tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

12192-1-AP targets Alpha Antichymotrypsin in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Antichymotrypsin fusion protein Ag2830
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
• Calculated Molecular Weight: 448 aa, 48 kDa
• Observed Molecular Weight: 44-70 kDa
• GenBank Accession Number: BC010530
• Gene Symbol: Alpha 1 Antichymotrypsin
• Gene ID (NCBI): 12
• RRID: AB_10344682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

Publications

Species	Application	Title
		Acta Neuropathol; Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS; Authors - Amr Aly
human	WB	Front Immunol; Screening and identification of tissue-infiltrating immune cells and genes for patients with emphysema phenotype of COPD; Authors - Di Wang
human	WB,IHC	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao
		Proteomics Clin Appl; Serum protein profiling in diffuse large B-cell lymphoma.; Authors - Jacques Riby
human,rat	WB	Food Funct; Sinensetin attenuates IL-1β-induced cartilage damage and ameliorates osteoarthritis by regulating SERPINA3; Authors - Zhendong Liu