SF3B3 Polyclonal antibody

SF3B3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14577-1-AP

Synonyms

KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130



Tested Applications

Positive WB detected inJurkat cells, mouse heart tissue, human brain tissue, rat brain tissue, rat heart tissue, HeLa cells
Positive IP detected inrat brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

14577-1-AP targets SF3B3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3B3 fusion protein Ag5980
Full Name splicing factor 3b, subunit 3, 130kDa
Calculated molecular weight 136 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC003146
Gene symbol SF3B3
Gene ID (NCBI) 23450
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

Protocols

Product Specific Protocols
WB protocol for SF3B3 antibody 14577-1-APDownload protocol
IHC protocol for SF3B3 antibody 14577-1-APDownload protocol
IF protocol for SF3B3 antibody 14577-1-APDownload protocol
IP protocol for SF3B3 antibody 14577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.

Authors - Coltri Patricia P
humanWB

RNA

Rearrangements within human spliceosomes captured after exon ligation.

Authors - Ilagan Janine O JO
humanWB

Cell Rep

SF3B1 Association with Chromatin Determines Splicing Outcomes.

Authors - Nir Kfir
humanWB

Cancer Res

PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.

Authors - Yi-Zi Zheng
humanWB

Nucleic Acids Res

Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Authors - Ruibing Chen

Genome Res

RNA structure replaces the need for U2AF2 in splicing.

Authors - Chien-Ling Lin