|Positive WB detected in||Jurkat cells, mouse heart tissue, human brain tissue, rat brain tissue, rat heart tissue, HeLa cells|
|Positive IP detected in||rat brain tissue|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14577-1-AP targets SF3B3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SF3B3 fusion protein Ag5980|
|Full Name||splicing factor 3b, subunit 3, 130kDa|
|Calculated molecular weight||136 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC003146|
|Gene ID (NCBI)||23450|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.
|Product Specific Protocols|
|WB protocol for SF3B3 antibody 14577-1-AP||Download protocol|
|IHC protocol for SF3B3 antibody 14577-1-AP||Download protocol|
|IF protocol for SF3B3 antibody 14577-1-AP||Download protocol|
|IP protocol for SF3B3 antibody 14577-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.
Rearrangements within human spliceosomes captured after exon ligation.
SF3B1 Association with Chromatin Determines Splicing Outcomes.
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.
Nucleic Acids Res
Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.
RNA structure replaces the need for U2AF2 in splicing.