SGOL2 Polyclonal antibody, PBS Only

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  WB analysis using 30867-1-AP (same clone as 30867-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30867-1-AP (SGOL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30867-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30867-1-AP (same clone as 30867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30867-1-AP (SGOL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 30867-1-AP (same clone as 30867-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SGOL2 antibody (30867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30867-1-PBS in a different storage buffer formulation.

SGOL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 30867-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

30867-1-PBS targets SGOL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGOL2 fusion protein Ag29656
• Full Name: shugoshin-like 2 (S. pombe)
• Calculated Molecular Weight: 1265 aa, 145 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC092412
• Gene Symbol: SGOL2
• Gene ID (NCBI): 151246
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q562F6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SGOL2 (Shugoshin 2, also known as SGO2), which is tightly involved in the cell cycle process, has been reported to protect cohesion, sustain the linkage of chromosomes, and regulate kinetochore-microtubule attachment in meiosis and mitosis (PMID: 21273764). SGOL2 has been reported as a differentially expressed gene in various types of cancer, including glioma, hepatocellular cancer, and endometrial cancer (PMID: 36566018).