SGOL2 Polyclonal antibody

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  WB analysis using 30867-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30867-1-AP (SGOL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 30867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30867-1-AP (SGOL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 30867-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SGOL2 antibody (30867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SGOL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30867-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ25211, SGO2, SGOL2, Shugoshin 2, Shugoshin like 2, shugoshin like 2 (S. pombe), TRIPIN

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, U2OS cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30867-1-AP targets SGOL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGOL2 fusion protein Ag29656
• Full Name: shugoshin-like 2 (S. pombe)
• Calculated Molecular Weight: 1265 aa, 145 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC092412
• Gene Symbol: SGOL2
• Gene ID (NCBI): 151246
• RRID: AB_3669770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q562F6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SGOL2 (Shugoshin 2, also known as SGO2), which is tightly involved in the cell cycle process, has been reported to protect cohesion, sustain the linkage of chromosomes, and regulate kinetochore-microtubule attachment in meiosis and mitosis (PMID: 21273764). SGOL2 has been reported as a differentially expressed gene in various types of cancer, including glioma, hepatocellular cancer, and endometrial cancer (PMID: 36566018).