SHC1 Polyclonal antibody

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  WB analysis using 12496-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12496-1-AP

  WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells.

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  WB analysis of HeLa using 12496-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12496-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12496-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 12496-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12496-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12496-1-AP targets SHC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHC1 fusion protein Ag3165
• Full Name: SHC (Src homology 2 domain containing) transforming protein 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa, 52 kDa, 46 kDa
• GenBank Accession Number: BC014158
• Gene Symbol: SHC1
• Gene ID (NCBI): 6464
• RRID: AB_2187889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29353
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Publications

Species	Application	Title
mouse	WB	Exp Cell Res; LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway.; Authors - Hao-Ke Li
human	WB,IF	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang
mouse	IHC	Biomed Pharmacother; Buparlisib and ponatinib inhibit aggressiveness of cholangiocarcinoma cells via suppression of IRS1-related pathway by targeting oxidative stress resistance; Authors - Waleeporn Kaewlert