SHC Polyclonal antibody
SHC Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF,ELISA
Conjugate
Unconjugated
Cat no : 12496-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
12496-1-AP targets SHC in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SHC fusion protein Ag3165 |
Full Name | SHC (Src homology 2 domain containing) transforming protein 1 |
Calculated molecular weight | 66 kDa |
Observed molecular weight | 66 kDa, 52 kDa, 46 kDa |
GenBank accession number | BC014158 |
Gene symbol | SHC1 |
Gene ID (NCBI) | 6464 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SHC antibody 12496-1-AP | Download protocol |
IF protocol for SHC antibody 12496-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Cell Res LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway. |