SHC Polyclonal antibody

• Featured Product
• KD/KO Validated

SHC Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12496-1-AP

Synonyms

SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA

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Validation Data Gallery

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  WB analysis of HeLa using 12496-1-AP

  WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells.

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  WB analysis using 12496-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12496-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 12496-1-AP

  Immunofluorescent analysis of HepG2 cells, using SHC1 antibody 12496-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12496-1-AP targets SHC in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHC fusion protein Ag3165
• Full Name: SHC (Src homology 2 domain containing) transforming protein 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa, 52 kDa, 46 kDa
• GenBank Accession Number: BC014158
• Gene Symbol: SHC1
• Gene ID (NCBI): 6464
• RRID: AB_2187889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Publications

Species	Application	Title
mouse	WB	Exp Cell Res; LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway.; Authors - Hao-Ke Li
human	WB,IF	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang