|Positive WB detected in
|Jurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12496-1-AP targets SHC in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|SHC fusion protein Ag3165
|SHC (Src homology 2 domain containing) transforming protein 1
|Calculated molecular weight
|Observed molecular weight
|66 kDa, 52 kDa, 46 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.
Exp Cell Res
LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway.
HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis