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SHC Polyclonal antibody

SHC Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 12496-1-AP

Synonyms

SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12496-1-AP targets SHC in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHC fusion protein Ag3165
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa, 52 kDa, 46 kDa
GenBank accession numberBC014158
Gene symbol SHC1
Gene ID (NCBI) 6464
RRIDAB_2187889
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Protocols

Product Specific Protocols
WB protocol for SHC antibody 12496-1-APDownload protocol
IF protocol for SHC antibody 12496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Cell Res

LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway.

Authors - Hao-Ke Li
humanWB,IF

Sci Adv

HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis

Authors - Qiwei Jiang