SHC1 Polyclonal antibody

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SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12496-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

• 

  WB analysis using 12496-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 12496-1-AP

  WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 12496-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 12496-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 12496-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 12496-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12496-1-AP targets SHC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3165

  Product name: Recombinant human SHC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 175-474 aa of BC014158

  Sequence: RACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAAPGAARPTAPNAQTPSHLGATLPVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLDKARQAVGGAGPPNPAINGSAPRDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWFHGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVERKL

• Full Name: SHC (Src homology 2 domain containing) transforming protein 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa, 52 kDa, 46 kDa
• GenBank Accession Number: BC014158
• Gene Symbol: SHC1
• Gene ID (NCBI): 6464
• RRID: AB_2187889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29353
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Publications

What published studies show

The SHC1 Polyclonal antibody (Cat# 12496-1-AP) has 6 total citations. These appear in journals including Science Advances, Biomedical Pharmacotherapy, Journal of Medicinal Chemistry, Experimental Cell Research, PLoS One, and Oncogene. The research fields span oncology and cancer biology, covering EGFR-RAS-ERK signaling in tumorigenesis, cholangiocarcinoma, RET inhibitor discovery, myoblast differentiation via FGFR1 signaling, lung adenocarcinoma prognosis, and ferroptosis resistance in pancreatic cancer. Applications include WB, IF, and IHC in human and mouse samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang
mouse	IHC	Biomed Pharmacother; Buparlisib and ponatinib inhibit aggressiveness of cholangiocarcinoma cells via suppression of IRS1-related pathway by targeting oxidative stress resistance; Authors - Waleeporn Kaewlert
human,mouse	WB	J Med Chem; Discovery of CN-3 as a Next-Generation RET Inhibitor Potently Overcoming Multiple Mutations.; Authors - Zi-Xuan Wang
mouse	WB	Exp Cell Res; LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway.; Authors - Hao-Ke Li
human	IHC	PLoS One; Construction and validation of a prognostic model based on mitochondria-associated endoplasmic reticulum membranes gene signature in LUAD patients.; Authors - Qichen Zhang
human	WB	Oncogene; DDR1 sustains ferroptosis resistance in pancreatic ductal adenocarcinoma via SLC40A1-mediated iron homeostasis; Authors - Chao Song