Validation Data Gallery
|Positive WB detected in
|LNCaP cells, fetal human brain tissue, pig heart tissue, pig brain tissue, rat heart tissue, rat brain tissue, mouse brain tissue, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
|Positive IHC detected in
|human kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66410-1-Ig targets SIRT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|human, mouse, rat, pig
|human, mouse, rat
|Host / Isotype
|Mouse / IgG1
|SIRT2 fusion protein Ag7756
|sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.
A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation.
CNS Neurosci Ther
Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage.
SIRT2 promotes cell proliferation and migration through mediating ERK1/2 activation and lactosylceramide accumulation in prostate cancer
Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation
Development of a multiplex assay to assess activated p300/CBP in circulating prostate tumor cells
Nicotinamide riboside alleviates cisplatin-induced peripheral neuropathy via SIRT2 activation.