SIRT2 Monoclonal antibody

• Featured Product
• KD/KO Validated

SIRT2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1D8G10

Cat no : 66410-1-Ig

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

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Validation Data Gallery

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  WB analysis using 66410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of MCF-7 using 66410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66410-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66410-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66410-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66410-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66410-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66410-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66410-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66410-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 66410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 66410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HepG2 using 66410-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66410-1-Ig(SIRT2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, fetal human brain tissue, pig heart tissue, pig brain tissue, rat heart tissue, rat brain tissue, mouse brain tissue, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

66410-1-Ig targets SIRT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT2 fusion protein Ag7756
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC003547
• Gene Symbol: SIRT2
• Gene ID (NCBI): 22933
• RRID: AB_2881782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.

Publications

Species	Application	Title
mouse	WB	Redox Biol; A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation.; Authors - David Siegel ; KD Validated
rat	WB,IHC	CNS Neurosci Ther; Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage.; Authors - Liu Yang
human	WB	Prostate; SIRT2 promotes cell proliferation and migration through mediating ERK1/2 activation and lactosylceramide accumulation in prostate cancer; Authors - Rui Lin ; KD Validated
human	WB	PLoS Pathog; Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation; Authors - Dan-Ni Wang ; KD Validated
human	IF	Oncotarget; Development of a multiplex assay to assess activated p300/CBP in circulating prostate tumor cells; Authors - Mikolaj Filon
mouse	IHC	Neurooncol Adv; Nicotinamide riboside alleviates cisplatin-induced peripheral neuropathy via SIRT2 activation.; Authors - Scarlett Acklin