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  • KD/KO Validated

SIRT2 Monoclonal antibody

SIRT2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D8G10

Cat no : 66410-1-Ig

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2



Tested Applications

Positive WB detected inLNCaP cells, fetal human brain tissue, pig heart tissue, pig brain tissue, rat heart tissue, rat brain tissue, mouse brain tissue, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66410-1-Ig targets SIRT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT2 fusion protein Ag7756
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Calculated molecular weight 43 kDa
Observed molecular weight 37-45 kDa
GenBank accession numberBC003547
Gene symbol SIRT2
Gene ID (NCBI) 22933
RRIDAB_2881782
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.

Protocols

Product Specific Protocols
WB protocol for SIRT2 antibody 66410-1-IgDownload protocol
IHC protocol for SIRT2 antibody 66410-1-IgDownload protocol
IF protocol for SIRT2 antibody 66410-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation.

Authors - David Siegel
  • KD Validated
ratWB,IHC

CNS Neurosci Ther

Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage.

Authors - Liu Yang
humanWB

Prostate

SIRT2 promotes cell proliferation and migration through mediating ERK1/2 activation and lactosylceramide accumulation in prostate cancer

Authors - Rui Lin
  • KD Validated
humanWB

PLoS Pathog

Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation

Authors - Dan-Ni Wang
  • KD Validated
humanIF

Oncotarget

Development of a multiplex assay to assess activated p300/CBP in circulating prostate tumor cells

Authors - Mikolaj Filon
mouseIHC

Neurooncol Adv

Nicotinamide riboside alleviates cisplatin-induced peripheral neuropathy via SIRT2 activation.

Authors - Scarlett Acklin