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- KD/KO Validated
SIRT2 Monoclonal antibody
SIRT2 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1D8G10
Cat no : 66410-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, rat brain tissue, mouse brain tissue, rat heart tissue, pig brain tissue, pig heart tissue, fetal human brain tissue |
Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 4 publications below |
IHC | See 2 publications below |
Product Information
66410-1-Ig targets SIRT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SIRT2 fusion protein Ag7756 |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 37-45 kDa |
GenBank accession number | BC003547 |
Gene symbol | SIRT2 |
Gene ID (NCBI) | 22933 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. SIRT2 exists various isoforms and the molecular weight of isoforms are 35kDa, 40 kDa, and 42 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for SIRT2 antibody 66410-1-Ig | Download protocol |
IHC protocol for SIRT2 antibody 66410-1-Ig | Download protocol |
IF protocol for SIRT2 antibody 66410-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Redox Biol A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation.
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CNS Neurosci Ther Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage. | ||
Prostate SIRT2 promotes cell proliferation and migration through mediating ERK1/2 activation and lactosylceramide accumulation in prostate cancer
| ||
PLoS Pathog Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation
| ||
Neurooncol Adv Nicotinamide riboside alleviates cisplatin-induced peripheral neuropathy via SIRT2 activation. |