Tested Applications
| Positive WB detected in | LNCaP cells, fetal human brain tissue, pig heart tissue, pig brain tissue, rat heart tissue, rat brain tissue, mouse brain tissue, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
66410-1-Ig targets SIRT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SIRT2 fusion protein Ag7756 Predict reactive species |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 37-45 kDa |
| GenBank Accession Number | BC003547 |
| Gene Symbol | SIRT2 |
| Gene ID (NCBI) | 22933 |
| RRID | AB_2881782 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8IXJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT2 antibody 66410-1-Ig | Download protocol |
| IHC protocol for SIRT2 antibody 66410-1-Ig | Download protocol |
| IF protocol for SIRT2 antibody 66410-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation.
| ||
Cell Death Dis FBXO31 is upregulated by METTL3 to promote pancreatic cancer progression via regulating SIRT2 ubiquitination and degradation | ||
CNS Neurosci Ther Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage. | ||
Prostate SIRT2 promotes cell proliferation and migration through mediating ERK1/2 activation and lactosylceramide accumulation in prostate cancer
| ||
PLoS Pathog Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation
| ||
Oncotarget Development of a multiplex assay to assess activated p300/CBP in circulating prostate tumor cells |
