SIRT2 Monoclonal antibody

SIRT2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D8G10

Cat no : 66410-1-Ig

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2



Tested Applications

Positive WB detected inMCF-7 cells, rat brain tissue, mouse brain tissue, rat heart tissue, pig brain tissue, pig heart tissue, fetal human brain tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66410-1-Ig targets SIRT2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT2 fusion protein Ag7756
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Calculated molecular weight 43 kDa
Observed molecular weight 37-45 kDa
GenBank accession numberBC003547
Gene symbol SIRT2
Gene ID (NCBI) 22933
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. SIRT2 exists various isoforms and the molecular weight of isoforms are 35kDa, 40 kDa, and 42 kDa.

Protocols

Product Specific Protocols
WB protocol for SIRT2 antibody 66410-1-IgDownload protocol
IHC protocol for SIRT2 antibody 66410-1-IgDownload protocol
IF protocol for SIRT2 antibody 66410-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

A redox-mediated conformational change in NQO1 controls binding to microtubules and α-tubulin acetylation.

Authors - David Siegel
  • KD Validated