CoraLite® Plus 488-conjugated SIRT2 Monoclonal antibody
SIRT2 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D8G10
Cat no : CL488-66410
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66410 targets SIRT2 in IF applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SIRT2 fusion protein Ag7756 |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 37-45 kDa |
GenBank accession number | BC003547 |
Gene symbol | SIRT2 |
Gene ID (NCBI) | 22933 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. SIRT2 exists various isoforms and the molecular weight of isoforms are 35kDa, 40 kDa, and 42 kDa.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 SIRT2 antibody CL488-66410 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |