WB analysis of HeLa using 17466-1-AP (same clone as 17466-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 17466-1-AP (same clone as 17466-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
A549 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 17466-1-AP (same clone as 17466-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
HepG2 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 17466-1-AP (same clone as 17466-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 17466-1-AP (same clone as 17466-1-PBS)
IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 17466-1-AP (same clone as 17466-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 17466-1-AP (same clone as 17466-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 17466-1-AP (same clone as 17466-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17466-1-AP (SKAR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17466-1-AP (SKAR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis of HeLa using 17466-1-AP (same clone as 17466-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 17466-1-AP (same clone as 17466-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 17466-1-AP (same clone as 17466-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 17466-1-AP (same clone as 17466-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human stomach using 17466-1-AP (same clone as 17466-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 17466-1-AP (same clone as 17466-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IP Figures
IP experiment of mouse brain using 17466-1-AP (same clone as 17466-1-PBS)
IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HeLa using 17466-1-AP (same clone as 17466-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17466-1-AP (SKAR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17466-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SKAR Polyclonal antibody
Catalog Number
17466-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, IP, Indirect ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
