SKAR Polyclonal antibody

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• SKAR Polyclonal antibody (17466-1-AP)

SKAR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17466-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

POLDIP3, KIAA1649, p46, PDIP3, PDIP46

• 

  WB analysis using 17466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 17466-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 17466-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 17466-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17466-1-AP (SKAR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 17466-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKAR antibody (17466-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17466-1-AP targets SKAR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11338

  Product name: Recombinant human POLDIP3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-229 aa of BC019643

  Sequence: MADISLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAREMLNSRKQQTTVPQKPRQVADAREKISLKRSSPAAFINPPIGTVTPALKLTKTIQNLYDLDEDDDGIASVPTKQMKFAASGGFLHHMAGLSSSKLSMSKALPLTKVVQNDAYTAPALPSSIRTKALPVLTSPGT

• Full Name: polymerase (DNA-directed), delta interacting protein 3
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC019643
• Gene Symbol: SKAR
• Gene ID (NCBI): 84271
• RRID: AB_2166620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BY77
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The antibody 17466-1-AP targets POLDIP3 (SKAR). It has a total of 3 citations appearing in Oncotarget, Scientific Reports, and American Journal of Cancer Research. The associated research fields include DNA polymerase δ activation, TDP-43 aggregation and its effects on protein expression, and mTORC1 signaling in hepatocellular carcinoma.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncotarget; PDIP46 (DNA polymerase δ interacting protein 46) is an activating factor for human DNA polymerase δ.; Authors - Xiaoxiao Wang
human	WB,IF	Sci Rep; TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.; Authors - S Prpar Mihevc
human	WB	Am J Cancer Res; Inhibition of mTORC1 signaling sensitizes hepatocellular carcinoma cells to glycolytic stress.; Authors - Xin Zhao