Validation Data Gallery
|Positive WB detected in
|HuH-7 cells, COLO 320 cells, Caco-2 cells, rat kidney tissue, SH-SY5Y cells, Neuro-2a cells
|Positive IHC detected in
|mouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse small intestine tissue, mouse brain tissue
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:50-1:500
|IF : 1:400-1:1600
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20507-1-AP targets DMT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, chicken, pig
|Host / Isotype
|Rabbit / IgG
|DMT1 fusion protein Ag14314
|solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
|Calculated molecular weight
|568 aa, 62 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that play a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including the brain, kidney, testis, duodenum, and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.
Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.
Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.
Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.
Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.
Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.
Apolipoprotein E deficiency induces a progressive increase in tissue iron contents with age in mice.