|Positive WB detected in||Caco-2 cells, HEK-293 cells, Neuro-2a cells, COLO 320 cells, rat kidney tissue, SH-SY5Y cells|
|Positive IHC detected in||mouse brain tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20507-1-AP is DMT1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DMT1 fusion protein Ag14314|
|Full Name||solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2|
|Calculated molecular weight||568 aa, 62 kDa|
|Observed molecular weight||60-70 kDa, 75-100 kDa|
|GenBank accession number||BC002592|
|Gene ID (NCBI)||4891|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.
Aging (Albany NY)
Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice.
J Biol Chem
The intestinal metal transporter ZIP14 maintains systemic manganese homeostasis.
The Effects of the Combination of Oral Lactoferrin and Iron Injection on Iron Homestasis, Antioxidative Abilities and Cytokines Activities of Suckling Piglets.
Cell Death Dis
Cystathionine β-synthase (CBS) deficiency suppresses erythropoiesis by disrupting expression of heme biosynthetic enzymes and transporter.
Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.
Oxid Med Cell Longev
Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo.