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DMT1 Polyclonal antibody

DMT1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20507-1-AP

Synonyms

DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT 1, DMT1, NRAMP 2, NRAMP2, SLC11A2



Tested Applications

Positive WB detected inCaco-2 cells, HEK-293 cells, Neuro-2a cells, COLO 320 cells, rat kidney tissue, SH-SY5Y cells
Positive IHC detected inmouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20507-1-AP is DMT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mosue, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMT1 fusion protein Ag14314
Full Name solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Calculated molecular weight 568 aa, 62 kDa
Observed molecular weight 60-70 kDa, 75-100 kDa
GenBank accession numberBC002592
Gene symbol SLC11A2
Gene ID (NCBI) 4891
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

Protocols

Product Specific Protocols
WB protocol for DMT1 antibody 20507-1-APDownload protocol
IHC protocol for DMT1 antibody 20507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice.

Authors - Meng-Wan Zhang
mouseWB

J Biol Chem

The intestinal metal transporter ZIP14 maintains systemic manganese homeostasis.

Authors - Ivo Florin Scheiber
pigWB

Animals (Basel)

The Effects of the Combination of Oral Lactoferrin and Iron Injection on Iron Homestasis, Antioxidative Abilities and Cytokines Activities of Suckling Piglets.

Authors - Ping Hu
mouseWB

Cell Death Dis

Cystathionine β-synthase (CBS) deficiency suppresses erythropoiesis by disrupting expression of heme biosynthetic enzymes and transporter.

Authors - Peng Zhao
ratWB

Transl Res

Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.

Authors - Guang Yang
humanWB

Oxid Med Cell Longev

Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo.

Authors - Saideng Lu