• Featured Product
  • KD/KO Validated

NKCC2 Polyclonal antibody

NKCC2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18970-1-AP

Synonyms

BSC1, NKCC2, SLC12A1



Tested Applications

Positive WB detected inHEK-293 cells, 37°C incubated mouse kidney tissue
Positive IHC detected inhuman kidney tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse Embryonic kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18970-1-AP is NKCC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKCC2 fusion protein Ag5325
Full Name solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Calculated molecular weight 121 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC040138
Gene symbol SLC12A1
Gene ID (NCBI) 6557
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NKCC2 antibody 18970-1-APDownload protocol
IHC protocol for NKCC2 antibody 18970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Tissue Eng Part A

PMID: 32312181

Authors - Harold Dean Love
human

Cell Stem Cell

Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk.

Authors - Sara E Howden
mouseIF

JCI Insight

Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome.

Authors - Sierra S Marable
mouseIF

Physiol Genomics

Gene expression profile and injury sites in mice treated with Shiga toxin 2 and lipopolysaccharide as a Shiga toxin-associated hemolytic uremic syndrome model.

Authors - Yohei Kume
humanIF

Dev Biol

Reduced Abd-B Hox function during kidney development results in lineage infidelity.

Authors - Bliss Magella
human,mouseIHC,IF

Eur J Pharmacol

Na+-K+-2Cl- cotransporter 2 located in the human and murine gastric mucosa is involved in secretagogue-induced gastric acid secretion and is downregulated in lipopolysaccharide-treated mice.

Authors - Li-Fei Zheng