SLC25A12 Monoclonal antibody

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  WB analysis of LNCaP using 67467-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67467-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67467-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 67467-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC25A12 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67467-1-Ig

Clone No.1F2B10

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

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Tested Applications

• Positive WB detected in: LNCaP cells, A431 cells, Jurkat cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67467-1-Ig targets SLC25A12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC25A12 fusion protein Ag13576
• Full Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Calculated Molecular Weight: 75 kDa, 63 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC016932
• Gene Symbol: SLC25A12
• Gene ID (NCBI): 8604
• RRID: AB_2882696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75746
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Nat Commun; Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces; Authors - Jinghua Fang