SLC25A12 Monoclonal antibody, PBS Only (Detector)

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  WB analysis of LNCaP using 67467-1-Ig (same clone as 67467-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67467-1-Ig (same clone as 67467-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67467-1-Ig (same clone as 67467-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50650-2

  Cytometric bead array standard curve of MP50650-2, SLC25A12 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67467-4-PBS. Detection antibody: 67467-1-PBS. Standard:Ag13576. Range: 1.563-100 ng/mL.

SLC25A12 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 67467-1-PBS

Clone No.1F2B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

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Product Information

67467-1-PBS targets SLC25A12 as part of a matched antibody pair:

MP50650-2: 67467-4-PBS capture and 67467-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC25A12 fusion protein Ag13576
• Full Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Calculated Molecular Weight: 75 kDa, 63 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC016932
• Gene Symbol: SLC25A12
• Gene ID (NCBI): 8604
• RRID: AB_2882696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75746
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).