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GLUT2 Polyclonal antibody
GLUT2 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 20436-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, HEK 293 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 15 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 20436-1-AP is GLUT2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, Lasiopodomys brandtii, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GLUT2 fusion protein Ag14204 |
| Full Name | solute carrier family 2 (facilitated glucose transporter), member 2 |
| Calculated molecular weight | 351 aa, 39 kDa |
| Observed molecular weight | 60-70 kDa, 38-45 kDa |
| GenBank accession number | BC060041 |
| Gene symbol | SLC2A2 |
| Gene ID (NCBI) | 6514 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glucose transporter 2 (GLUT2), also known as solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2), is a transporter protein regulating glucose transport across cell membranes in an insulin-independent manner.
What is the molecular weight of GLUT2? Is GLUT2 post-translationally modified?
GLUT2 can be N-glycosylated. The molecular weight of mature glycosylated GLUT2 transporter is between 55 and 65 kDa, but a minor 38-kDa form is often detected in hepatocytes that may represent a non-glycosylated or proteolytic product (PMID: 12101013).
What is the subcellular localization of GLUT2?
Glucose transporters, including GLUT2, are multiple-pass integral membrane proteins. GLUT2 is present at the plasma membrane but has also been reported to be present at intracellular membranes, including the endoplasmic reticulum (PMID: 12101013).
What molecules can be transported by GLUT2?
Although the main substrate of GLUT2 transport is glucose, it can also transport galactose, mannose, and fructose.
What is the tissue expression pattern of GLUT2?
GLUT2 is expressed in many cell types. High expression of GLUT2 is observed in hepatocytes, where GLUT2 mediates glucose uptake and release regulating glucose levels in blood, in intestinal cells, where it regulates glucose transport at the basolateral membrane and to a lesser extent from the apical membrane, and in the kidney proximal tubule, where it is involved in glucose reabsorption and is present at the basolateral membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GLUT2 antibody 20436-1-AP | Download protocol |
| IHC protocol for GLUT2 antibody 20436-1-AP | Download protocol |
| IP protocol for GLUT2 antibody 20436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells. | ||
Autophagy The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance. | ||
J Food Biochem Pea-derived peptides, VLP, LLP, VA, and LL, improve insulin resistance in HepG2 cells via activating IRS-1/PI3K/AKT and blocking ROS-mediated p38MAPK signaling. | ||
J Food Sci Chlorogenic Acid Stimulates the Thermogenesis of Brown Adipocytes by Promoting the Uptake of Glucose and the Function of Mitochondria. | ||
PLoS One Effects of canagliflozin on growth and metabolic reprograming in hepatocellular carcinoma cells: Multi-omics analysis of metabolomics and absolute quantification proteomics (iMPAQT). | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iram (Verified Customer) (09-17-2020) | Excellent GLUT-2 Antibody. Giving a very good results for WB and IF.
|
FH Pradeep (Verified Customer) (09-17-2020) | Very good antibody for western blot. I checked in INS-1 cell line.
|
