Tested Applications
| Positive WB detected in | Caco-2 cells, mouse kidney tissue, HEK 293 cells, HepG2 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 67 publications below |
| IHC | See 15 publications below |
| IF | See 13 publications below |
Product Information
20436-1-AP targets GLUT2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, sheep, lasiopodomys brandtii |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GLUT2 fusion protein Ag14204 Predict reactive species |
| Full Name | solute carrier family 2 (facilitated glucose transporter), member 2 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 60-70 kDa, 38-45 kDa |
| GenBank Accession Number | BC060041 |
| Gene Symbol | GLUT2 |
| Gene ID (NCBI) | 6514 |
| RRID | AB_2750600 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11168 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glucose transporter 2 (GLUT2), also known as solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2), is a transporter protein regulating glucose transport across cell membranes in an insulin-independent manner.
What is the molecular weight of GLUT2? Is GLUT2 post-translationally modified?
GLUT2 can be N-glycosylated. The molecular weight of mature glycosylated GLUT2 transporter is between 55 and 65 kDa, but a minor 38-kDa form is often detected in hepatocytes that may represent a non-glycosylated or proteolytic product (PMID: 12101013).
What is the subcellular localization of GLUT2?
Glucose transporters, including GLUT2, are multiple-pass integral membrane proteins. GLUT2 is present at the plasma membrane but has also been reported to be present at intracellular membranes, including the endoplasmic reticulum (PMID: 12101013).
What molecules can be transported by GLUT2?
Although the main substrate of GLUT2 transport is glucose, it can also transport galactose, mannose, and fructose.
What is the tissue expression pattern of GLUT2?
GLUT2 is expressed in many cell types. High expression of GLUT2 is observed in hepatocytes, where GLUT2 mediates glucose uptake and release regulating glucose levels in blood, in intestinal cells, where it regulates glucose transport at the basolateral membrane and to a lesser extent from the apical membrane, and in the kidney proximal tubule, where it is involved in glucose reabsorption and is present at the basolateral membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GLUT2 antibody 20436-1-AP | Download protocol |
| IHC protocol for GLUT2 antibody 20436-1-AP | Download protocol |
| IP protocol for GLUT2 antibody 20436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Transl Med USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl | ||
Cell Mol Gastroenterol Hepatol Patient-Derived Mutant Forms of NFE2L2/NRF2 Drive Aggressive Murine Hepatoblastomas. | ||
Mol Metab T1R2 receptor-mediated glucose sensing in the upper intestine potentiates glucose absorption through activation of local regulatory pathways. | ||
Metabolism Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Balawant (Verified Customer) (07-25-2022) | This antibody I used and it is working great. I will recommend this antibody for Immunoblting.
|
FH Susmita (Verified Customer) (04-13-2022) | This antibody is working nicely for my WB
|
FH Boyan (Verified Customer) (08-24-2021) | Not conclusive. This antibody primarily recognized a band at ~ 90 kd, which did not respond to a Glut2 siRNA treatment. It also weakly recognized a band at ~ 70 kd, which responded to de-glycosylation treatment.
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FH Iram (Verified Customer) (09-17-2020) | Excellent GLUT-2 Antibody. Giving a very good results for WB and IF.
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FH Pradeep (Verified Customer) (09-17-2020) | Very good antibody for western blot. I checked in INS-1 cell line.
|
