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GLUT2 Polyclonal antibody

GLUT2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20436-1-AP

Synonyms

GLUT 2, GLUT2, SLC2A2



Tested Applications

Positive WB detected inrat liver tissue, HEK 293 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20436-1-AP is GLUT2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity human, Lasiopodomys brandtii, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLUT2 fusion protein Ag14204
Full Name solute carrier family 2 (facilitated glucose transporter), member 2
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight 60-70 kDa, 38-45 kDa
GenBank accession numberBC060041
Gene symbol SLC2A2
Gene ID (NCBI) 6514
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucose transporter 2 (GLUT2), also known as solute carrier family 2, facilitated glucose transporter member 2 (SLC2A2), is a transporter protein regulating glucose transport across cell membranes in an insulin-independent manner.

 

What is the molecular weight of GLUT2? Is GLUT2 post-translationally modified?

GLUT2 can be N-glycosylated. The molecular weight of mature glycosylated GLUT2 transporter is between 55 and 65 kDa, but a minor 38-kDa form is often detected in hepatocytes that may represent a non-glycosylated or proteolytic product (PMID: 12101013).

 

What is the subcellular localization of GLUT2?

Glucose transporters, including GLUT2, are multiple-pass integral membrane proteins. GLUT2 is present at the plasma membrane but has also been reported to be present at intracellular membranes, including the endoplasmic reticulum (PMID: 12101013).

 

What molecules can be transported by GLUT2?

Although the main substrate of GLUT2 transport is glucose, it can also transport galactose, mannose, and fructose.

 

What is the tissue expression pattern of GLUT2?

GLUT2 is expressed in many cell types. High expression of GLUT2 is observed in hepatocytes, where GLUT2 mediates glucose uptake and release regulating glucose levels in blood, in intestinal cells, where it regulates glucose transport at the basolateral membrane and to a lesser extent from the apical membrane, and in the kidney proximal tubule, where it is involved in glucose reabsorption and is present at the basolateral membrane.


Protocols

Product Specific Protocols
WB protocol for GLUT2 antibody 20436-1-APDownload protocol
IHC protocol for GLUT2 antibody 20436-1-APDownload protocol
IP protocol for GLUT2 antibody 20436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell Metab

High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.

Authors - Chun-Mei Hu
humanWB

Autophagy

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Authors - Xing Huang
humanWB

J Food Biochem

Pea-derived peptides, VLP, LLP, VA, and LL, improve insulin resistance in HepG2 cells via activating IRS-1/PI3K/AKT and blocking ROS-mediated p38MAPK signaling.

Authors - Yan Zhu
mouseWB

J Food Sci

Chlorogenic Acid Stimulates the Thermogenesis of Brown Adipocytes by Promoting the Uptake of Glucose and the Function of Mitochondria.

Authors - Xue Han
humanWB

PLoS One

Effects of canagliflozin on growth and metabolic reprograming in hepatocellular carcinoma cells: Multi-omics analysis of metabolomics and absolute quantification proteomics (iMPAQT).

Authors - Dan Nakano
HumanWB

J Mol Endocrinol

PMID: 27920093

Authors - Chen-Tian Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (09-17-2020)

Excellent GLUT-2 Antibody. Giving a very good results for WB and IF.

  • Applications: Western Blot,Flow Cytometry
  • Primary Antibody Dilution: 1:2000 for western and 1:200 for IF
  • Cell Tissue Type: Human colon cancer
FH

Pradeep (Verified Customer) (09-17-2020)

Very good antibody for western blot. I checked in INS-1 cell line.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: INS-1