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GLUT3 Polyclonal antibody

GLUT3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20403-1-AP

Synonyms

GLUT 3, GLUT3, SLC2A3



Tested Applications

Positive WB detected inHEK-293 cells, U-251 cells, Jurkat cells, HeLa cells, C6 cells, mouse brain tissue, rat brain tissue, HepG2 cells
Positive IHC detected inmouse testis tissue, human lung cancer tissue, human breast cancer tissue, human placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20403-1-AP targets GLUT3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLUT3 fusion protein Ag14203
Full Name solute carrier family 2 (facilitated glucose transporter), member 3
Calculated Molecular Weight 496 aa, 54 kDa
Observed Molecular Weight 48-60 kDa
GenBank Accession NumberBC039196
Gene Symbol SLC2A3
Gene ID (NCBI) 6515
RRIDAB_10694437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose transporter 3 (GLUT3), also known as solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3), is a transporter protein regulating glucose transport across cell membranes and is a primary glucose transporter in neurons.

What is the molecular weight of GLUT3? Is GLUT3 post-translationally modified?

The molecular weight of GLUT3 transporter is 49-52 kDa and depends on the glycosylation pattern, which is tissue-specific (PMID: 9253355 and 9889355). GLUT3 can be N-glycosylated.

What is the subcellular localization of GLUT3?

Glucose transporters, including GLUT3, are multiple-pass integral membrane proteins. GLUT3 is present at the plasma membrane but is also a subject of recycling between the plasma membrane and endosomes. 

What molecules can be transported by GLUT3?

Although the main substrate of GLUT3 transport is glucose, it can also transport mannose, galactose, and xylose.

What is the tissue expression pattern of GLUT3?

GLUT1 and GLUT3 are important for the transport of glucose into the central nervous system. While GLUT1 transports glucose through blood vessels and into astrocytes, GLUT3 is predominantly expressed in neurons, being their main glucose transporter (PMID: 9302083), and in testis (PMID: 18577699).


Protocols

Product Specific Protocols
WB protocol for GLUT3 antibody 20403-1-APDownload protocol
IHC protocol for GLUT3 antibody 20403-1-APDownload protocol
IF protocol for GLUT3 antibody 20403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Neuron

Sympathetic nerve-enteroendocrine L cell communication modulates GLP-1 release, brain glucose utilization, and cognitive function

Authors - Wenran Ren
humanWB

Mol Cancer

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Authors - Bing Yao
humanWB

Cell Death Differ

A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.

Authors - Xiaoyu Qi
humanWB

Autophagy

Newcastle disease virus degrades SIRT3 via PINK1-PRKN-dependent mitophagy to reprogram energy metabolism in infected cells.

Authors - Yabin Gong
mouseWB

Cell Rep

16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult mice

Authors - Alexandria Béland-Millar
humanWB

EMBO Rep

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

Authors - Xiuying Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

balawant (Verified Customer) (12-25-2023)

This antibody is very specific and has no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1200
  • Cell Tissue Type: Renal Cancer cell line caki-1 and caki-2
FH

Hua (Verified Customer) (02-03-2023)

This antibody works ok. There is one strong non-specific band around 40 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2, mouse liver, and mouse brain tissues
GLUT3 Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, and mouse brain tissues (Cat no:20403-1-AP)