Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue, U-251 cells|
|Positive IHC detected in||mouse testis tissue, human lung cancer tissue, human breast cancer tissue, human placenta tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20403-1-AP is GLUT3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GLUT3 fusion protein Ag14203|
|Full Name||solute carrier family 2 (facilitated glucose transporter), member 3|
|Calculated molecular weight||496 aa, 54 kDa|
|Observed molecular weight||48-60 kDa|
|GenBank accession number||BC039196|
|Gene ID (NCBI)||6515|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glucose transporter 3 (GLUT3), also known as solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3), is a transporter protein regulating glucose transport across cell membranes and is a primary glucose transporter in neurons.
What is the molecular weight of GLUT3? Is GLUT3 post-translationally modified?
The molecular weight of GLUT3 transporter is 49-52 kDa and depends on the glycosylation pattern, which is tissue-specific (PMID: 9253355 and 9889355). GLUT3 can be N-glycosylated.
What is the subcellular localization of GLUT3?
Glucose transporters, including GLUT3, are multiple-pass integral membrane proteins. GLUT3 is present at the plasma membrane but is also a subject of recycling between the plasma membrane and endosomes.
What molecules can be transported by GLUT3?
Although the main substrate of GLUT3 transport is glucose, it can also transport mannose, galactose, and xylose.
What is the tissue expression pattern of GLUT3?
GLUT1 and GLUT3 are important for the transport of glucose into the central nervous system. While GLUT1 transports glucose through blood vessels and into astrocytes, GLUT3 is predominantly expressed in neurons, being their main glucose transporter (PMID: 9302083), and in testis (PMID: 18577699).
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