SLC35B4 Recombinant antibody, PBS Only (Capture)

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  WB analysis of DU 145 using 83527-3-RR (same clone as 83527-3-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 83527-3-RR (SLC35B4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83527-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83527-3-RR (same clone as 83527-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Slc35b4 Recombinant Antibody (83527-3-RR, Clone:240332F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83527-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00497-1

  Cytometric bead array standard curve of MP00497-1, SLC35B4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83527-3-PBS. Detection antibody: 83527-2-PBS. Standard: Ag22669. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83527-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83527-3-RR against Human SLC35B4 were performed. The affinity constant is below 1 pM.

SLC35B4 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 83527-3-PBS

Clone No.240332F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4

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Product Information

83527-3-PBS targets SLC35B4 as part of a matched antibody pair:

MP00497-1: 83527-3-PBS capture and 83527-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC35B4 fusion protein Ag22669
• Full Name: solute carrier family 35, member B4
• GenBank Accession Number: BC008413
• Gene Symbol: SLC35B4
• Gene ID (NCBI): 84912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q969S0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.