SLC8A2 Polyclonal antibody

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  IHC staining of rat kidney using 31437-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31437-1-AP (SLC8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 31437-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 31437-1-AP (SLC8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat cerebellum using 31437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using SLC8A2 antibody (31437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC8A2 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 31437-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, IF-P, ELISA

Synonyms

Sodium/calcium exchanger 2, NCX2, KIAA1087

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Tested Applications

• Positive IHC detected in: rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat cerebellum tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31437-1-AP targets SLC8A2 in IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC8A2 fusion protein Ag34438
• Full Name: solute carrier family 8 (sodium/calcium exchanger), member 2
• Calculated Molecular Weight: 921 aa, 100 kDa
• GenBank Accession Number: NM_015063
• Gene Symbol: SLC8A2
• Gene ID (NCBI): 6543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9UPR5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC8A2, or solute carrier family 8 member 2, belongs to the SLC8 gene family, which encodes sodium-calcium exchangers (NCX). These proteins are part of the CaCA (Ca2+/Cation Antiporter) superfamily and play a significant role in regulating Ca2+-dependent events in many cell types. SLC8A2 has been implicated in various diseases, such as heart failure, arrhythmia, cerebral ischemia, hypertension, diabetes, and muscle dystrophy.