SLD5 Polyclonal antibody, PBS Only

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  WB analysis of mouse thymus using 13315-1-AP (same clone as 13315-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13315-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 13315-1-AP (same clone as 13315-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13315-1-AP (SLD5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13315-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13315-1-AP (same clone as 13315-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SLD5 antibody (13315-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13315-1-PBS in a different storage buffer formulation.

SLD5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 13315-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GINS4, GINS complex subunit 4, DNA replication complex GINS protein SLD5, N-terminally processed

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Product Information

13315-1-PBS targets SLD5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLD5 fusion protein Ag4158
• Full Name: GINS complex subunit 4 (Sld5 homolog)
• Calculated Molecular Weight: 223 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC027454
• Gene Symbol: SLD5
• Gene ID (NCBI): 84296
• RRID: AB_2109671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRT9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SLD5 (DNA replication complex GINS protein SLD5) is also named as GINS4 (GINS complex subunit 4). SLD5 is required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PMID: 17417653). SLD5 forms a GINS complex with PSF1, PSF2 and PSF3, which is essential for the initiation of DNA replication in lower eukaryotes (PMID: 24244394). SLD5 is a member of the GINS complex, essential for DNA replication in eukaryotes. It has been reported that SLD5 is involved in early embryogenesis in the mouse, and cell cycle progression and genome integrity in Drosophila (PMID: 27499248).