SMARCE1 Monoclonal antibody

SMARCE1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

CloneNo.

1F3D6

Cat no : 66182-1-Ig

Synonyms

BAF57, BRG1 associated factor 57, SMARCE1

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Validation Data Gallery

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  WB analysis of Jurkat using 66182-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 66182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HEK-293 using 66182-1-Ig

  Immunofluorescent analysis of HEK-293 cells using 66182-1-Ig (SMARCE1 antibody) at dilution of 1:50 and FITC-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66182-1-Ig targets SMARCE1 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMARCE1 fusion protein Ag1161
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007082
• Gene Symbol: SMARCE1
• Gene ID (NCBI): 6605
• RRID: AB_2881577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.