ChromoTek SNAP/CLIP-tag-Trap Agarose beads

Validation Data Gallery

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  Pull-down of SNAP-tag protein with using SNAP/CLIP-tag-Trap Agarose.I: Input, FT: Flow-through, B: Bound.

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

Cat No. wta

Publications(3)

Specificity

SNAP/CLIP-tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Synonyms

SNAP/CLIP-tag-Trap, SNAP/CLIP-tag, SNAP/CLIP, SNAP, human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT), SNAP-tag and CLIP-tag, SNAP-tag, CLIP-tag, CLIP

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Product Information

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

• Description: Immunoprecipitation of SNAP-tag and CLIP-tag fusion proteins and their interacting factors with anti-SNAP-tag and CLIP-tag Nanobody conjugated to beads.

  • SNAP/CLIP-tag-Trap immunoprecipitates SNAP-tag- and Clip-tag- fusion proteinseven when already covalently bound to ligands such as dyes, biotin etc.

  • Bound SNAP- or CLIP-tag fusion proteins can be eluted without protease

  • SNAP/CLIP-tag-Trap can be used for affinity purification

  • Structure and function are characterized

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: SNAP-tag, CLIP-tag
• Binding capacity: 7.5-10 µg of recombinant SNAP/CLIP-tag per 25 µL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 nM
• Compatibility with mass spectrometry: The SNAP/CLIP-tag®-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827598
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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Publications

Application	Title
IP	Nat Chem Biol; Systematic discovery of biomolecular condensate-specific protein phosphorylation.; Authors - Sindhuja Sridharan
IP	J Histochem Cytochem; Immunocytochemical Labeling of Rhabdomeric Proteins in Drosophila Photoreceptor Cells Is Compromised by a Light-dependent Technical Artifact.; Authors - Krystina Schopf
IP	PLoS Genet; Structured and disordered regions of Ataxin-2 contribute differently to the specificity and efficiency of mRNP granule formation; Authors - Arnas Petrauskas