ChromoTek SNAP/CLIP-tag-Trap Agarose beads
SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.
Specificity
SNAP-tag, CLIP-tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : wta
Synonyms
Validation Data Gallery
Product Information
SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 nM |
Specificity/Target | SNAP-tag and CLIP-tag |
Coupled VHH | Recombinant, monoclonal anti-SNAP/ CLIP-tag single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Binding capacity | 7.5-10 µg of recombinant SNAP/CLIP-tag per 25 µL bead slurry |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% EtOH |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. |
Documentation
SDS |
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SDS SNAP- CLIP-tag?-Trap Agarose (PDF) |
Protocol |
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Protocol SNAP-CLIP-tag?-Trap Agarose (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) (PDF) |
Publications
Application | Title |
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J Histochem Cytochem Immunocytochemical Labeling of Rhabdomeric Proteins in Drosophila Photoreceptor Cells Is Compromised by a Light-dependent Technical Artifact. |