ChromoTek SNAP/CLIP-tag-Trap Agarose beads, kit

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

Specificity

SNAP-tag, CLIP-tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Unconjugated

Cat no : wtak

Synonyms

SNAP/CLIP-tag-Trap, SNAP/CLIP-tag, SNAP/CLIP, SNAP, human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT), SNAP-tag and CLIP-tag, SNAP-tag, CLIP-tag, CLIP



Product Information

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 1 nM
Specificity/TargetSNAP-tag and CLIP-tag
Coupled VHHRecombinant, monoclonal anti-SNAP/ CLIP-tag single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 90 µm (cross-linked 4% agarose beads)
Binding capacity7.5-10 µg of recombinant SNAP/CLIP-tag per 25 µL bead slurry
Elution bufferSDS sample buffer
0.1 mM citric acid pH 3.0
Storage Buffer20% EtOH
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Kit components

ComponentDescription
SNAP/CLIP-tag-Trap Agarose beads20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution