ChromoTek SNAP/CLIP-tag-Trap Agarose beads, kit
SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.
Cat no : wtak
Synonyms
Validation Data Gallery
Product Information
SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 nM |
Specificity/Target | SNAP-tag and CLIP-tag |
Coupled VHH | Recombinant, monoclonal anti-SNAP/ CLIP-tag single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4% agarose beads) |
Binding capacity | 7.5-10 µg of recombinant SNAP/CLIP-tag per 25 µL bead slurry |
Elution buffer | SDS sample buffer 0.1 mM citric acid pH 3.0 |
Storage Buffer | 20% EtOH |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. |
Kit components
Component | Description |
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SNAP/CLIP-tag-Trap Agarose beads | 20 reactions (500 µL) |
Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
Wash buffer | Removal of unwanted proteins, peptides, etc. |
Dilution buffer | Dilution of cell lysate |
Elution buffer | For acidic elution |
Documentation
SDS |
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SDS SNAP- CLIP-tag?-Trap Agarose Kit (PDF) |
Protocol |
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Protocol SNAP- CLIP-tag?-Trap Agarose Kit (PDF) |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) (PDF) |