ChromoTek SNAP/CLIP-tag-Trap Agarose beads, kit

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

Specificity

SNAP-tag, CLIP-tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : wtak

Synonyms

SNAP/CLIP-tag-Trap, SNAP/CLIP-tag, SNAP/CLIP, SNAP, human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT), SNAP-tag and CLIP-tag, SNAP-tag, CLIP-tag, CLIP

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Validation Data Gallery

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  Pull-down of SNAP-tag protein with using SNAP/CLIP-tag-Trap Agarose. I: Input, FT: Flow-through, B: Bound.

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Product Information

SNAP/CLIP-tag®-Trap Agarose is an affinity reagent for IP of SNAP/CLIP-tag-fusion proteins. It consists of a SNAP/CLIP-tag Nanobody/ VHH, which is coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 nM
• Specificity/Target: SNAP-tag and CLIP-tag
• Coupled VHH: Recombinant, monoclonal anti-SNAP/ CLIP-tag single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4% agarose beads)
• Binding capacity: 7.5-10 µg of recombinant SNAP/CLIP-tag per 25 µL bead slurry
• Elution buffer: SDS sample buffer; 0.1 mM citric acid pH 3.0
• Storage Buffer: 20% EtOH
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Kit components

Component	Description
SNAP/CLIP-tag-Trap Agarose beads	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution