SNAP91 Polyclonal antibody, PBS Only

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  WB analysis using 14093-1-AP (same clone as 14093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14093-1-AP (SNAP91 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14093-1-AP (SNAP91 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 14093-1-AP (same clone as 14093-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SNAP91 antibody (14093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14093-1-PBS in a different storage buffer formulation.

SNAP91 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 14093-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Phosphoprotein F1-20, KIAA0656, Clathrin coat-associated protein AP180, Clathrin coat assembly protein AP180, 91 kDa synaptosomal-associated protein

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Product Information

14093-1-PBS targets SNAP91 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAP91 fusion protein Ag5224
• Full Name: synaptosomal-associated protein, 91kDa homolog (mouse)
• Calculated Molecular Weight: 92.5 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC060818
• Gene Symbol: SNAP91
• Gene ID (NCBI): 9892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60641
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SNAP91, also known as AP180 or CALM, is a clathrin-coat assembly protein that sequesters clathrin. SNAP91/AP180 is required for clathrin-dependent endocytosis and SV reformation (PMID: 26903854; PMID: 31843760).The AP180 N-terminal homology (ANTH) domains is crucially involved in membrane budding processes, along with and Epsin N-terminal homology (ENTH) domains (PMID: 29774507).