SNAP91 Polyclonal antibody
SNAP91 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14093-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
14093-1-AP targets SNAP91 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SNAP91 fusion protein Ag5224 |
Full Name | synaptosomal-associated protein, 91kDa homolog (mouse) |
Calculated Molecular Weight | 92.5 kDa |
Observed Molecular Weight | 160 kDa |
GenBank Accession Number | BC060818 |
Gene Symbol | SNAP91 |
Gene ID (NCBI) | 9892 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNAP91, also known as AP180 or CALM, is a clathrin-coat assembly protein that sequesters clathrin. SNAP91/AP180 is required for clathrin-dependent endocytosis and SV reformation (PMID: 26903854; PMID: 31843760).The AP180 N-terminal homology (ANTH) domains is crucially involved in membrane budding processes, along with and Epsin N-terminal homology (ENTH) domains (PMID: 29774507).
Protocols
Product Specific Protocols | |
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WB protocol for SNAP91 antibody 14093-1-AP | Download protocol |
IHC protocol for SNAP91 antibody 14093-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Emerg Microbes Infect CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis |