|Positive WB detected in||HeLa cells, human brain tissue, mouse skin tissue, mouse brain tissue, HepG2 cells, mouse cerebellum tissue, mouse liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10275-1-AP targets SNX17 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SNX17 fusion protein Ag0355|
|Full Name||sorting nexin 17|
|Calculated molecular weight||53 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC002524|
|Gene ID (NCBI)||9784|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.
|Product Specific Protocols|
|WB protocol for SNX17 antibody 10275-1-AP||Download protocol|
|IHC protocol for SNX17 antibody 10275-1-AP||Download protocol|
|IF protocol for SNX17 antibody 10275-1-AP||Download protocol|
|IP protocol for SNX17 antibody 10275-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
Adaptor protein sorting nexin 17 interacts with the scavenger receptor FEEL-1/stabilin-1 and modulates its expression on the cell surface.
Suppression of Membranous LRP5 Recycling, Wnt/β-Catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P.
J Mol Biol
Sorting nexin 31 binds multiple β integrin cytoplasmic domains and regulates β1 integrin surface levels and stability.
SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling.
SNX17 protects the heart from doxorubicin-induced cardiotoxicity by modulating LMOD2 degradation.
J Cell Biol
TFEB controls retromer expression in response to nutrient availability.