Validation Data Gallery
|Positive WB detected in||HeLa cells, human brain tissue, mouse skin tissue, mouse brain tissue, HepG2 cells, mouse cerebellum tissue, mouse liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10275-1-AP targets SNX17 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SNX17 fusion protein Ag0355|
|Full Name||sorting nexin 17|
|Calculated molecular weight||53 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC002524|
|Gene ID (NCBI)||9784|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.
|Product Specific Protocols|
|WB protocol for SNX17 antibody 10275-1-AP||Download protocol|
|IHC protocol for SNX17 antibody 10275-1-AP||Download protocol|
|IF protocol for SNX17 antibody 10275-1-AP||Download protocol|
|IP protocol for SNX17 antibody 10275-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
Adaptor protein sorting nexin 17 interacts with the scavenger receptor FEEL-1/stabilin-1 and modulates its expression on the cell surface.
Suppression of Membranous LRP5 Recycling, Wnt/β-Catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P.
Life Sci Alliance
New insights into the phosphorylation of the threonine motif of the β1 integrin cytoplasmic domain.
J Mol Biol
Sorting nexin 31 binds multiple β integrin cytoplasmic domains and regulates β1 integrin surface levels and stability.
Front Cardiovasc Med
The Role of Sorting Nexin 17 in Cardiac Development.
Regulation of Cell Migration and β1 Integrin Trafficking by the Endosomal Adaptor GGA3.