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  • KD/KO Validated

SNX17 Polyclonal antibody

SNX17 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10275-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

KIAA0064, Sorting nexin-17

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inK-562 cells, HeLa cells, human brain tissue, mouse skin tissue, mouse brain tissue, mouse cerebellum tissue, RAW 264.7 cells, SK-N-SH cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10275-1-AP targets SNX17 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0355

Product name: Recombinant human SNX17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 17-226 aa of BC002524

Sequence: GSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYA

Predict reactive species
Full Name sorting nexin 17
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002524
Gene Symbol SNX17
Gene ID (NCBI) 9784
RRIDAB_2192394
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15036
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled-coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin and may function in the intracellular trafficking of P-selectin.

Protocols

Product Specific Protocols
WB protocol for SNX17 antibody 10275-1-APDownload protocol
IHC protocol for SNX17 antibody 10275-1-APDownload protocol
IP protocol for SNX17 antibody 10275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide conversion mechanism for exit from endosomes.

Authors - Katharina Ketel
  • KD Validated
mouseWB

Nat Cell Biol

Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.

Authors - B?ttcher Ralph Thomas RT
  • KD Validated
humanWB

Nat Cell Biol

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.

Authors - Kerrie E McNally
humanWB

Nat Cell Biol

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Authors - Chuchu Zhou
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
mouseWB

Proc Natl Acad Sci U S A

LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.

Authors - Björn Schorch
  • KD Validated
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