SNX25 Polyclonal antibody, PBS Only

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  WB analysis using 13294-1-AP (same clone as 13294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13294-1-AP (same clone as 13294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 13294-1-AP (same clone as 13294-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 13294-1-AP (same clone as 13294-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 13294-1-AP (same clone as 13294-1-PBS)

  IP result of anti-SNX25 (IP:13294-1-AP, 4ug; Detection:13294-1-AP 1:2000) with Raji cells lysate 1600 ug. This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13294-1-AP (same clone as 13294-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX25 antibody (13294-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13294-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SNX25 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 13294-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Sorting nexin-25, sorting nexin 25, SBBI31, MSTP043

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Product Information

13294-1-PBS targets SNX25 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX25 fusion protein Ag3995
• Full Name: sorting nexin 25
• Calculated Molecular Weight: 98 kDa
• Observed Molecular Weight: 98-105 kDa, 65 kDa
• GenBank Accession Number: BC029868
• Gene Symbol: SNX25
• Gene ID (NCBI): 83891
• RRID: AB_2192549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H3E2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-β receptors. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation.This antibody can recognize two isoforms: 98-105 kDa and 65 kDa.