SOX4 Recombinant antibody, PBS Only (Capture)

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  WB analysis of MCF-7 using 83660-1-RR (same clone as 83660-1-PBS)

  WB result of SOX4 antibody (83660-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX4 transfected MCF-7 cells. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

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  WB analysis using 83660-1-RR (same clone as 83660-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00619-1

  Sandwich ELISA standard curve of MP00619-1, Human SOX4 Recombinant Matched Antibody Pair - PBS only. 83660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26583. 83660-3-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

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  Sample test of MP00619-1

  The mean SOX4 concentration was determined to be 16.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

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  FC experiment of SH-SY5Y using 83660-1-RR (same clone as 83660-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83660-1-RR (same clone as 83660-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00619-1

  Cytometric bead array standard curve of MP00619-1, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-3-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83660-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

SOX4 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83660-1-PBS

Clone No.240625E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

240625E3, CSS10, EVI16, Transcription factor SOX-4

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Product Information

83660-1-PBS targets SOX4 as part of a matched antibody pair:

MP00619-1: 83660-1-PBS capture and 83660-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00619-2: 83660-1-PBS capture and 83660-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SOX4 fusion protein Ag26583
• Full Name: SRY (sex determining region Y)-box 4
• Calculated Molecular Weight: 474 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC072668
• Gene Symbol: SOX4
• Gene ID (NCBI): 6659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q06945
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa.