SPRY4 Polyclonal antibody, PBS Only

• 

  WB analysis of A375 using 22765-1-AP (same clone as 22765-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 22765-1-AP (SPRY4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 22765-1-AP (same clone as 22765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22765-1-AP (SPRY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 22765-1-AP (same clone as 22765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22765-1-AP (SPRY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22765-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SPRY4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 22765-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Spry-4, Spry 4, sprouty homolog 4 (Drosophila), Protein sprouty homolog 4, HH17

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Product Information

22765-1-PBS targets SPRY4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPRY4 fusion protein Ag18574
• Full Name: sprouty homolog 4 (Drosophila)
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC125095
• Gene Symbol: SPRY4
• Gene ID (NCBI): 81848
• RRID: AB_2879163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C004
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SPRY4, also named as Protein sprouty homolog 4, is a 299 amino acid protein, which contains one SPR (sprouty) domain and belongs to the sprouty family. SPRY4 Suppresses the INS receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. SPRY4 has a cellular function to regulate the actin cytoskeleton, independent of its inhibitory activity on the Ras/MAP kinase signalling. SPRY1, SPRY2, SPRY3, SPRY4, are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway.