SPTB Polyclonal antibody, PBS Only

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  WB analysis using 26936-1-AP (same clone as 26936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26936-1-AP (SPTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26936-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 26936-1-AP (same clone as 26936-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SPTB antibody (26936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26936-1-PBS in a different storage buffer formulation.

SPTB Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 26936-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Spectrin beta chain, erythrocytic, Beta-I spectrin, Beta I spectrin

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Product Information

26936-1-PBS targets SPTB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPTB fusion protein Ag25614
• Full Name: spectrin, beta, erythrocytic
• Observed Molecular Weight: 246 kDa
• GenBank Accession Number: BC136284
• Gene Symbol: SPTB
• Gene ID (NCBI): 6710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11277
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Beta I spectrin also known as SPTB, SPTB1, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTB have been associated with Elliptocytosis 3, spherocytosis type 2, and neonatal hemolytic anemia(PMID: 8018926, 8102379, 9005995).