SPTB Polyclonal antibody

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  WB analysis using 26936-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26936-1-AP (SPTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26936-1-AP (SPTB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 26936-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using SPTB antibody (26936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SPTB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26936-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Beta I spectrin, Beta-I spectrin, Spectrin beta chain, erythrocytic

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Tested Applications

• Positive WB detected in: K-562 cells, mouse heart tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26936-1-AP targets SPTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25614

  Product name: Recombinant human SPTB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1800-1900 aa of BC136284

  Sequence: HRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLV

• Full Name: spectrin, beta, erythrocytic
• Observed Molecular Weight: 246 kDa
• GenBank Accession Number: BC136284
• Gene Symbol: SPTB
• Gene ID (NCBI): 6710
• RRID: AB_3669575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11277
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta I spectrin also known as SPTB, SPTB1, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTB have been associated with Elliptocytosis 3, spherocytosis type 2, and neonatal hemolytic anemia(PMID: 8018926, 8102379, 9005995).