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- KD/KO Validated
SREBF1 Polyclonal antibody
SREBF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (4)
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Cat no : 14088-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, L02 cells, MCF-7 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human kidney tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 7 publications below|
|WB||See 132 publications below|
|IHC||See 18 publications below|
|IF||See 13 publications below|
|IP||See 1 publications below|
|CoIP||See 1 publications below|
|ChIP||See 1 publications below|
14088-1-AP targets SREBF1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, bovine, chicken, goat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SREBF1 fusion protein Ag5219|
|Full Name||sterol regulatory element binding transcription factor 1|
|Calculated molecular weight||1177 aa, 125 kDa|
|Observed molecular weight||125 kDa, 68 kDa|
|GenBank accession number||BC063281|
|Gene ID (NCBI)||6720|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.
|Product Specific Protocols|
|WB protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|IHC protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|IF protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|IP protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A metastasis map of human cancer cell lines.
Divergent transcriptional regulation of astrocyte reactivity across disorders.
TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.
CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.
Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Isha (Verified Customer) (05-18-2022)
Antibody worked perfect at 1:1000 dilution for W.B. and 1:500 for IF. And for chIP 3ug/ml worked best.