|Positive WB detected in||HeLa cells, L02 cells, MCF-7 cells|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human kidney tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14088-1-AP targets SREBF1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||bovine, chicken, goat, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SREBF1 fusion protein Ag5219|
|Full Name||sterol regulatory element binding transcription factor 1|
|Calculated molecular weight||1177 aa, 125 kDa|
|Observed molecular weight||125 kDa, 68 kDa|
|GenBank accession number||BC063281|
|Gene ID (NCBI)||6720|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125 kDa precursor form and the 68 kDa mature form of human SREBF1.
|Product Specific Protocols|
|WB protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|IHC protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|IF protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|IP protocol for SREBF1 antibody 14088-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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