SREBF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SREBF2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 28212-1-AP

Synonyms

bHLHd2, SREBF2, SREBP 2, SREBP2

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Validation Data Gallery

• 

  WB analysis using 28212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 1 publications below

Product Information

28212-1-AP targets SREBF2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SREBF2 fusion protein Ag28205
• Full Name: sterol regulatory element binding transcription factor 2
• Calculated molecular weight: 124 kDa
• Observed molecular weight: 73-75 kDa, 120-130 kDa
• GenBank accession number: BC056158
• Gene symbol: SREBF2
• Gene ID (NCBI): 6721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. SREBF2 exists two isoform with molecular weight 124 kDa and 73 kDa.

Publications

Species	Application	Title
human	WB	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng
human,mouse	WB	Cancer Commun (Lond); PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.; Authors - Weixing Dai
human	WB	Front Cell Dev Biol; The Chromatin Remodeling Protein BRG1 Regulates SREBP Maturation by Activating SCAP Transcription in Hepatocytes.; Authors - Ming Kong
human	WB	Front Oncol; A Novel c-MET-Targeting Antibody-Drug Conjugate for Pancreatic Cancer.; Authors - Yangbing Jin
mouse	WB	Biochim Biophys Acta Mol Basis Dis; The role of AMPKα2 in the HFD-induced nonalcoholic steatohepatitis.; Authors - Xuejiao Zhang
human	WB	Front Pharmacol; SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis.; Authors - Tingting Zhu