SREBF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SREBF2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 28212-1-AP

Synonyms

bHLHd2, SREBF2, SREBP 2, SREBP2

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Validation Data Gallery

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  WB analysis using 28212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28212-1-AP (SREBF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HUVEC cells, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

28212-1-AP targets SREBF2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SREBF2 fusion protein Ag28205
• Full Name: sterol regulatory element binding transcription factor 2
• Calculated molecular weight: 124 kDa
• Observed molecular weight: 73-75 kDa, 120-130 kDa
• GenBank accession number: BC056158
• Gene symbol: SREBF2
• Gene ID (NCBI): 6721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. SREBF2 exists two isoform with molecular weight 124 kDa and 73 kDa.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Brahma Related Gene 1 (Brg1) Regulates Cellular Cholesterol Synthesis by Acting as a Co-factor for SREBP2.; Authors - Zhiwen Fan ; KD Validated
human	WB,IF	Cell Death Dis; LPCAT1 reprogramming cholesterol metabolism promotes the progression of esophageal squamous cell carcinoma.; Authors - Mingyue Tao
mouse	WB	Adv Sci (Weinh); Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.; Authors - Zhiyuan Lu ; KD Validated
human	WB	Biol Pharm Bull; Inhibition of stearoyl-CoA desaturase 1 potentiates anti-tumor activity of amodiaquine in non-small cell lung cancer.; Authors - Xiaolei Hu ; KD Validated
mouse	WB	Lett Appl Microbiol; Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis.; Authors - R Yang
human	WB	Nutr Cancer; Osthole Increases the Sensitivity of Liver Cancer to Sorafenib by Inhibiting Cholesterol Metabolism.; Authors - Ke Fan