• Featured Product
  • KD/KO Validated

SREBF2 Polyclonal antibody

SREBF2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28212-1-AP

Synonyms

bHLHd2, SREBF2, SREBP 2, SREBP2



Tested Applications

Positive WB detected inHUVEC cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28212-1-AP targets SREBF2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SREBF2 fusion protein Ag28205
Full Name sterol regulatory element binding transcription factor 2
Calculated Molecular Weight 124 kDa
Observed Molecular Weight73-75 kDa, 120-130 kDa
GenBank Accession NumberBC056158
Gene Symbol SREBF2
Gene ID (NCBI) 6721
RRIDAB_2881091
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. SREBF2 exists two isoform with molecular weight 124 kDa and 73 kDa.

Protocols

Product Specific Protocols
WB protocol for SREBF2 antibody 28212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Redox Biol

Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation

Authors - Youzi Kong
  • KD Validated
humanWB

Mol Metab

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Authors - Han Zeng
humanWB

Front Cell Dev Biol

Brahma Related Gene 1 (Brg1) Regulates Cellular Cholesterol Synthesis by Acting as a Co-factor for SREBP2.

Authors - Zhiwen Fan
  • KD Validated
mouseWB,IHC

Diabetes Metab J

Long Non-Coding RNA TUG1 Attenuates Insulin Resistance in Mice with Gestational Diabetes Mellitus via Regulation of the MicroRNA-328-3p/SREBP-2/ERK Axis

Authors - Xuwen Tang
human,mouseWB

Cancer Commun (Lond)

PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.

Authors - Weixing Dai
mouseWB

J Nutr Biochem

Maternal high-calorie diet feeding programs hepatic cholesterol metabolism and Abca1 promoter methylation in the early life of offspring

Authors - Ling Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shunbin (Verified Customer) (05-14-2024)

Not good. Only non specific bands detected

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: B cell lines
SREBF2 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:28212-1-AP)
FH

Shunbin (Verified Customer) (05-14-2024)

Many non specific bands, but no specific band can be identified

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
SREBF2 Antibody Western Blot validation (1:1000 dilution) in (Cat no:28212-1-AP)
FH

Bertrand (Verified Customer) (04-08-2024)

This is a Srebp2 antibody, however, the results from western blot analysis show that the product might not be specific to Srebf2...There are lots of non-specific bands and the uncleaved/immature protein should not be present in the nucleus of cells, yet it was there.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver homogenates
FH

Boyan (Verified Customer) (04-17-2023)

OK for WB, a specific but weak band was seen around 150 kd, but not 130 kd, which is shown on its booklet. The 130 kd band is a strong nonspecific band.

  • Applications: Western Blot