STAG1 Polyclonal antibody, PBS Only

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  WB analysis of human heart using 14015-1-AP (same clone as 14015-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 14015-1-AP (same clone as 14015-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

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  WB analysis using 14015-1-AP (same clone as 14015-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14015-1-AP (same clone as 14015-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 14015-1-AP (same clone as 14015-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14015-1-AP (STAG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

STAG1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14015-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1

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Product Information

14015-1-PBS targets STAG1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAG1 fusion protein Ag5123
• Full Name: stromal antigen 1
• Calculated Molecular Weight: 144 kDa
• Observed Molecular Weight: 155 kDa
• GenBank Accession Number: BC064699
• Gene Symbol: STAG1
• Gene ID (NCBI): 10274
• RRID: AB_2194759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVM7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.