STAT5A/B Polyclonal antibody

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  WB analysis of Daudi using 13179-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 13179-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13179-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of 3T3-L1 using 13179-1-AP

  IP result of anti-STAT5A/B (IP:13179-1-AP, 4ug; Detection:13179-1-AP 1:5000) with 3T3-L1 cells lysate 1360 ug.

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  IP experiment of K-562 using 13179-1-AP

  IP result of anti-STAT5A/B (IP:13179-1-AP, 4ug; Detection:13179-1-AP 1:5000) with K-562 cells lysate 2800 ug.

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  IHC staining of human breast cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13179-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13179-1-AP (STAT5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13179-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13179-1-AP (STAT5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13179-1-AP (STAT5A/B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 13179-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13179-1-AP (STAT5A antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of MCF-7 using 13179-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug STAT5A/B Polyclonal antibody (13179-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

STAT5A/B Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 13179-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

STAT5A, STAT5, MGF

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Tested Applications

• Positive WB detected in: K-562 cells, 3T3-L1 cells, Daudi cells, DU 145 cells, SGC-7901 cells
• Positive IP detected in: K-562 cells, 3T3-L1 cells
• Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

13179-1-AP targets STAT5A/B in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, cow
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAT5A/B fusion protein Ag3846
• Full Name: signal transducer and activator of transcription 5A
• Calculated Molecular Weight: 794 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC027036
• Gene Symbol: STAT5A
• Gene ID (NCBI): 6776
• RRID: AB_2196760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42229
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against 445-794 aa of human STAT5A, the homology between this segment and the corresponding segment of human STAT5B is 92%. It shows that this antibody can cross-react with STAT5B.

Publications

Species	Application	Title
human	WB	Front Immunol; Exhausted signature and regulatory network of NK cells in myasthenia gravis; Authors - Qing Zhang
human	WB	Front Immunol; Elevated levels of exogenous prolactin promote inflammation at the maternal-fetal interface via the JAK2/STAT5B signaling axis; Authors - Alycia Williams
rat	WB,IF	Chemosphere; MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.; Authors - Yuezhu Zhang ; KD Validated
human	WB	Endocrinology; A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration; Authors - Ruonan Li
human	IHC,WB	Front Oncol; In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer; Authors - Xiaodi Gong
rat	WB	Ecotoxicol Environ Saf; Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.; Authors - Yuezhu Zhang