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STAT5A Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 13179-1-AP

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Con: 31 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, FC, ELISA
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Images See all

STAT5A Antibody IHC human breast cancer tissue 13179-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13179-1-AP( STAT5A antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

STAT5A Antibody WB K-562 cells 13179-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP(STAT5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

STAT5A Antibody IP 3T3-L1 cells 13179-1-AP

IP Result of anti-STAT5A (IP:13179-1-AP, 4ug; Detection:13179-1-AP 1:1000) with 3T3-L1 cells lysate 3200ug.

STAT5A Antibody FC MCF-7 cells 13179-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug STAT5A antibody (13179-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells, 3T3-L1 cells, DU 145 cells
Positive IP detected in:
3T3-L1 cells
Positive IHC detected in:
human breast cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
STAT5A fusion protein Ag3846
Full name:
signal transducer and activator of transcription 5A
Calculated molecular weight:
794aa,92 kDa
Observed molecular weight:
90-95 kDa,50-55 kDa
GenBank accession number:
BC027036
Gene ID (NCBI):
6776
Gene symbol
STAT5A
Synonyms
MGF, STAT5, STAT5A

Background

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.


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