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  • KD/KO Validated

STK25 Polyclonal antibody

STK25 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25821-1-AP

Synonyms

SOK 1, SOK1, Ste20 like kinase, STK25, YSK1



Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25821-1-AP is STK25 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK25 fusion protein Ag22964
Full Name serine/threonine kinase 25 (STE20 homolog, yeast)
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC015793
Gene symbol STK25
Gene ID (NCBI) 10494
RRIDAB_2880255
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STK25 antibody 25821-1-APDownload protocol
IP protocol for STK25 antibody 25821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Cell Mol Gastroenterol Hepatol

Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease.

Authors - Emmelie Cansby
human,mouseWB

Cell Mol Gastroenterol Hepatol

Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways.

Authors - Yeshwant Kurhe
  • KD Validated
humanWB

J Exp Clin Cancer Res

STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.

Authors - Fan Wu
  • KD Validated
humanWB

Mol Carcinog

Downregulation of STK25 promotes autophagy via the Janus kinase 2/signal transducer and activator of transcription 3 pathway in colorectal cancer.

Authors - Jiangbo Chen
ratWB

Cell Mol Neurobiol

Lanthanum Chloride Induces Axon Abnormality Through LKB1-MARK2 and LKB1-STK25-GM130 Signaling Pathways.

Authors - Zeli Song
mouseWB

Biochem Biophys Res Commun

Loss of serine/threonine protein kinase 25 in retinal ganglion cells ameliorates high glucose-elicited damage through regulation of the AKT-GSK-3β/Nrf2 pathway.

Authors - Zhuolin Zhou
  • KD Validated