MST1 Monoclonal antibody

• Featured Product
• KD/KO Validated

MST1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse , Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

2G11C1

Cat no : 66663-1-Ig

Synonyms

DKFZp686A2068, KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 66663-1-Ig

  WB result of MST1 antibody (66663-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells.

• 

  WB analysis using 66663-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66663-1-Ig (MST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66663-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 66663-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MST1 antibody (66663-1-Ig, Clone: 2G11C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

66663-1-Ig targets MST1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MST1 fusion protein Ag17738
• Full Name: serine/threonine kinase 4
• Calculated Molecular Weight: 487 aa, 56 kDa
• Observed Molecular Weight: 52-56 kDa
• GenBank Accession Number: BC093768
• Gene Symbol: MST1
• Gene ID (NCBI): 6789
• RRID: AB_2882018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Publications

Species	Application	Title
human	WB	Biofactors; FERM domain-containing protein 6 exerts a tumor-inhibiting role in thyroid cancer by antagonizing oncogenic YAP1; Authors - Wei Wang
human	WB	J Biol Chem; Angiomotins stimulate LATS kinase autophosphorylation and act as scaffolds that promote Hippo signaling.; Authors - Sebastian Mana-Capelli
mouse	IHC	J Endocr Soc; Effect of Inactivation of Mst1 and Mst2 in the Mouse Adrenal Cortex; Authors - Nour Abou Nader
human		Biochem Pharmacol; A humanized Anti-YKL-40 antibody inhibits tumor development; Authors - Haihui Yu ; KD Validated
mouse	WB	Br J Pharmacol; Diminished nuclear-localized β-adrenoceptor signalling activates YAP to promote kidney fibrosis in diabetic nephropathy; Authors - Wenjing Xiang