STMN2 Monoclonal antibody

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  WB analysis of PC-12 using 67204-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67204-1-Ig

  WB result of STMN2 antibody (67204-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STMN2 transfected SH-SY5Y cells.

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  WB analysis of fetal human brain using 67204-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67204-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67204-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67204-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67204-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67204-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 67204-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (67204-1-Ig, Clone: 1F6C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

STMN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67204-1-Ig

Clone No.1F6C4

Publications(4)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

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Tested Applications

• Positive WB detected in: PC-12 cells, fetal human brain tissue, mouse brain tissue, pig brain tissue, rat brain tissue, SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

67204-1-Ig targets STMN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28990

  Product name: Recombinant human STMN2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-179 aa of BC006302

  Sequence: MAKTAMAYKEKMKELSMLSLICSCFYPEPRNINIYTYDDMEVKQINKRASGQAFELILKPPSPISEAPRTLASPKKKDLSLEEIQKKLEAAEERRKSQEAQVLKQLAEKREHEREVLQKALEENNNFSKMAEEKLILKMEQIKENREANLAAIIERLQEKERHAAEVRRNKELQVELSG

• Full Name: stathmin-like 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC006302
• Gene Symbol: STMN2
• Gene ID (NCBI): 11075
• RRID: AB_2882497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93045
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Neuron; Molecular pathways and diagnosis in spatially resolved Alzheimer's hippocampal atlas; Authors - Pan Wang
human	IF	Nature; A model of human neural networks reveals NPTX2 pathology in ALS and FTLD; Authors - Marian Hruska-Plochan
human	IF	Neuron; TDP-43 seeding induces cytoplasmic aggregation heterogeneity and nuclear loss of function of TDP-43; Authors - Jens Rummens
human	WB	bioRxiv; Tubulin Regulates the Stability and Localization of STMN2 by Binding Preferentially to Its Soluble Form; Authors - Xiang Deng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (06-15-2025)

Incubation ON at 4C, in 5% goat serum/PBST. Gives specific cytoplasmic/Golgi signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cells/human

STMN2 Antibody Immunofluorescence validation (1:1000 dilution) in SH-SY5Y cells/human (Cat no:67204-1-Ig)