CoraLite® Plus 488-conjugated STRA8 Monoclonal antibody

STRA8 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7E3

Cat no : CL488-68071

Synonyms

STRA8

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Validation Data Gallery

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  IF Staining of NCCIT using CL488-68071

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using CoraLite® Plus 488 STRA8 antibody (CL488-68071, Clone: 1D7E3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: NCCIT cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68071 targets STRA8 in IF applications and shows reactivity with Human, rabbit, mouse samples.

• Tested Reactivity: Human, rabbit, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STRA8 fusion protein Ag29680
• Full Name: stimulated by retinoic acid gene 8 homolog (mouse)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 43-60 kda
• GenBank Accession Number: NM_182489
• Gene Symbol: STRA8
• Gene ID (NCBI): 346673
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stimulated by retinoic acid 8 (Stra8), one of genes induced by retinoic acid (RA), is required for the meiotic initiation of male spermatogenesis. Stimulated by Retinoic Acid Gene 8 (STRA8) protein has been proposed as the molecular effector of Retinoic Acid (RA) involved in meiosis-inductive activity. Stra8 is a 45-kDa vertebrate-specific gene, without significant homologous proteins, expressed in the cytoplasm and nuclei of germ cells between mitosis and meiosis and presents exclusively in the embryonic and postnatal gonads of mammals. (PMID: 30106128, PMID: 31253359)