STRAP Polyclonal antibody

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  WB analysis of HEK-293 using 18277-1-AP

  WB result of STRAP antibody (18277-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells.

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  WB analysis using 18277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18277-1-AP (STRAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18277-1-AP

  IP result of anti-STRAP (IP:18277-1-AP, 4ug; Detection:18277-1-AP 1:2000) with HeLa cells lysate 2000ug.

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  IHC staining of human ovary tumor using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 18277-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STRAP antibody (18277-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

STRAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18277-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

18277-1-AP targets STRAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13085

  Product name: Recombinant human STRAP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC000162

  Sequence: MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHIVKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILSADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIKSFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA

• Full Name: serine/threonine kinase receptor associated protein
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC000162
• Gene Symbol: STRAP
• Gene ID (NCBI): 11171
• RRID: AB_2255489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y3F4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy ; KD Validated
human	WB,IP	EMBO J; The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.; Authors - Muhammad Riaz Khan ; KD Validated
human		Cancers (Basel); Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines.; Authors - Laura V Bownes ; KD Validated
human	WB	Front Oncol; STRAP as a New Therapeutic Target for Poor Prognosis of Pancreatic Ductal Adenocarcinoma Patients Mainly Caused by TP53 Mutation.; Authors - Shanshan Hu ; KD Validated
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy
human	WB,IF	Int J Mol Sci; SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability.; Authors - Miao Yu