|Positive IP detected in
|Positive IHC detected in
|human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|MCF-7 cells, A549 cells
|Positive FC detected in
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10329-1-AP targets SUMO1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|SUMO1 fusion protein Ag0414
|SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
|Calculated molecular weight
|Observed molecular weight
|10-12 kDa, 80-90 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).
1. What is the molecular weight of SUMO1?
The molecular weight of SUMO1 is 12 kDa.
2. What is the cellular localization of SUMO1?
SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).
3. What modifications is SUMO1 subjected to?
SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).
4. What is the function of SUMO1?
SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.
5. What is SUMO1's involvement in disease?
Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).
|Product Specific Protocols
|WB protocol for SUMO1 antibody 10329-1-AP
|IHC protocol for SUMO1 antibody 10329-1-AP
|IF protocol for SUMO1 antibody 10329-1-AP
|IP protocol for SUMO1 antibody 10329-1-AP
|Click here to view our Standard Protocols
XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility
Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.
Sensitive detection of intracellular sumoylation via SNAP tag-mediated translation and RNA polymerase-based amplification.
Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.
J Mol Cell Biol
SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.
Int J Mol Med
SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Zoe (Verified Customer) (10-08-2021)
Hadn’t previously had much luck with antibodies on my Tricky construct so was great to use this one!