SUMO1 Polyclonal antibody

SUMO1 Polyclonal Antibody for FC, IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10329-1-AP


DAP 1, GAP modifying protein 1, GMP1, OFC10, PIC1, Sentrin, SMT3, SMT3 homolog 3, SMT3C, SMT3H3, SUMO 1, SUMO1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, UBL1

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, A549 cells
Positive FC detected inA549 cells

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10329-1-AP targets SUMO1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUMO1 fusion protein Ag0414
Full Name SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Calculated molecular weight 12 kDa
Observed molecular weight 10-12 kDa, 80-90 kDa
GenBank accession numberBC006462
Gene symbol SUMO1
Gene ID (NCBI) 7341
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).


Product Specific Protocols
WB protocol for SUMO1 antibody 10329-1-APDownload protocol
IHC protocol for SUMO1 antibody 10329-1-APDownload protocol
IF protocol for SUMO1 antibody 10329-1-APDownload protocol
IP protocol for SUMO1 antibody 10329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility

Authors - Ming Kuang


Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.

Authors - Shuai Huang

Anal Chem

Sensitive detection of intracellular sumoylation via SNAP tag-mediated translation and RNA polymerase-based amplification.

Authors - Yong Yang


Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.

Authors - Xiaoqing Liu

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu

Int J Mol Med

SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary.

Authors - Jing Cao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Zoe (Verified Customer) (10-08-2021)

Hadn’t previously had much luck with antibodies on my Tricky construct so was great to use this one!

  • Applications: Western Blot