SUMO1 Monoclonal antibody

SUMO1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3B5B5

Reviews (1)

Cat no : 67559-1-Ig

Synonyms

DAP 1, GAP modifying protein 1, GMP1, OFC10, PIC1, Sentrin, SMT3, SMT3 homolog 3, SMT3C, SMT3H3, SUMO 1, SUMO1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, UBL1

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Validation Data Gallery

• 

  WB analysis using 67559-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67559-1-Ig (SUMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 67559-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (67559-1-Ig, Clone: 3B5B5 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:800-1:3200

Sample-dependent, check data in validation data gallery

Published Applications

• IP: See 1 publications below

Product Information

67559-1-Ig targets SUMO1 in WB, IP, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SUMO1 fusion protein Ag29402
• Full Name: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Calculated molecular weight: 12 kDa
• Observed molecular weight: 15-100 kDa
• GenBank accession number: BC006462
• Gene symbol: SUMO1
• Gene ID (NCBI): 7341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IP	Biomed Pharmacother; Hydrogen sulfide regulates SERCA2a SUMOylation by S-Sulfhydration of SENP1 to ameliorate cardiac systole-diastole function in diabetic cardiomyopathy; Authors - Shuo Peng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (05-29-2023)

It works well in IP assay in human white adipocytes.

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes