|Positive WB detected in||mouse kidney tissue, A549 cells, mouse lung tissue, human heart tissue, rat kidney tissue|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15666-1-AP targets SYNJ2BP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SYNJ2BP fusion protein Ag8130|
|Full Name||synaptojanin 2 binding protein|
|Calculated molecular weight||145 aa, 16 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC007704|
|Gene ID (NCBI)||55333|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. ARIP2 is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. ARIP2 may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).
|Product Specific Protocols|
|WB protocol for SYNJ2BP antibody 15666-1-AP||Download protocol|
|IHC protocol for SYNJ2BP antibody 15666-1-AP||Download protocol|
|IF protocol for SYNJ2BP antibody 15666-1-AP||Download protocol|
|IP protocol for SYNJ2BP antibody 15666-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Exp Clin Cancer Res
SYNJ2BP inhibits tumor growth and metastasis by activating DLL4 pathway in hepatocellular carcinoma.
SYNJ2BP promotes the degradation of PTEN through the lysosome-pathway and enhances breast tumor metastasis via PI3K/AKT/SNAI1 signaling.
Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.