SYNM Polyclonal antibody, PBS Only

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  WB analysis using 20735-1-AP (same clone as 20735-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. SYNM gene methylation in T-47D cells causes gene silencing. This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 20735-1-AP (same clone as 20735-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 20735-1-AP (same clone as 20735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 20735-1-AP (same clone as 20735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 20735-1-AP (same clone as 20735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20735-1-AP (same clone as 20735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 20735-1-AP (same clone as 20735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bladder using 20735-1-AP (same clone as 20735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20735-1-AP (same clone as 20735-1-PBS)

  Immunofluorescent analysis of HeLa cells using 20735-1-AP (SYNM antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

SYNM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20735-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

20735-1-PBS targets SYNM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: synemin, intermediate filament protein
• Calculated Molecular Weight: 173 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: NM_015286
• Gene Symbol: SYNM
• Gene ID (NCBI): 23336
• RRID: AB_11042328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15061
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SYNM, also named as DMN, KIAA0353 and SYN, belongs to the intermediate filament family. SYNM is a Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. The antibody can't recognize isoform 2 or 3 of SYNM.