SYNM Polyclonal antibody

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  WB analysis using 20735-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. SYNM gene methylation in T-47D cells causes gene silencing.

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  WB analysis of human skeletal muscle using 20735-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human bladder using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 20735-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20735-1-AP

  Immunofluorescent analysis of HeLa cells using 20735-1-AP (SYNM antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

SYNM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20735-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Synemin, DMN, Desmuslin

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Tested Applications

• Positive WB detected in: Y79 cells, human skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human bladder tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20735-1-AP targets SYNM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: synemin, intermediate filament protein
• Calculated Molecular Weight: 173 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: NM_015286
• Gene Symbol: SYNM
• Gene ID (NCBI): 23336
• RRID: AB_11042328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15061
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYNM, also named as DMN, KIAA0353 and SYN, belongs to the intermediate filament family. SYNM is a Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. The antibody can't recognize isoform 2 or 3 of SYNM.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	J Ovarian Res; A risk model of gene signatures for predicting platinum response and survival in ovarian cancer.; Authors - Siyu Chen
human	IF	bioRxiv; Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.; Authors - Madeline G Andrews