SYNPO Polyclonal antibody

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  WB analysis of mouse brain using 21064-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21064-1-AP (Synaptopodin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21064-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21064-1-AP (Synaptopodin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 21064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21064-1-AP (Synaptopodin antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21064-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21064-1-AP (Synaptopodin antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of rat brain using 21064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Synaptopodin antibody (21064-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 21064-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (21064-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYNPO Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 21064-1-AP

Publications(68)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Synaptopodin, snaptopodin, KIAA1029

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 31 publications below
• IHC: See 8 publications below
• IF: See 42 publications below

Product Information

21064-1-AP targets SYNPO in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYNPO fusion protein Ag15089
• Full Name: synaptopodin
• Calculated Molecular Weight: 929 aa, 99 kDa
• Observed Molecular Weight: 99-115 kDa
• GenBank Accession Number: BC146665
• Gene Symbol: SYNPO
• Gene ID (NCBI): 11346
• RRID: AB_10733120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3V7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Publications

Species	Application	Title
mouse	IF	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	IF	Biofabrication; A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts; Authors - Zhilin Dai
human	IF	Theranostics; Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.; Authors - Chunmei Xu
human,mouse	IF	Diabetes; Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.; Authors - Hua Qu
mouse	IF	Free Radic Biol Med; P2X7R/AKT/mTOR signaling mediates high glucose-induced decrease in podocyte autophagy; Authors - Cheng Qian
mouse	WB,IF	Mol Ther Nucleic Acids; Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.; Authors - Ziyang Wang