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Synaptopodin Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 21064-1-AP

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Con: 35 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Synaptopodin Antibody WB mouse brain tissue 21064-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 21064-1-AP(Synaptopodin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Synaptopodin Antibody IF Hela cells 21064-1-AP

Immunofluorescent analysis of HepG2 cells, using SYNPO antibody 21064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Synaptopodin Antibody IHC mouse brain tissue 21064-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21064-1-AP( Synaptopodin antibody at dilution of 1:200 (under 10x lens).

Synaptopodin Antibody IHC mouse brain tissue 21064-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21064-1-AP( Synaptopodin antibody at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, rat brain tissue
Positive IHC detected in:
mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells, HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Synaptopodin fusion protein Ag15089
Full name:
synaptopodin
Calculated molecular weight:
929aa,99 kDa
Observed molecular weight:
99-115 kDa
GenBank accession number:
BC146665
Gene ID (NCBI):
11346
Gene symbol
SYNPO
Synonyms
KIAA1029, synaptopodin, SYNPO

Background

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)


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