SYNPO Polyclonal antibody
Synaptopodin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21064-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 16 publications below |
| IHC | See 3 publications below |
| IF | See 15 publications below |
Product Information
The immunogen of 21064-1-AP is Synaptopodin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synaptopodin fusion protein Ag15089 |
| Full Name | synaptopodin |
| Calculated molecular weight | 929 aa, 99 kDa |
| Observed molecular weight | 99-115 kDa |
| GenBank accession number | BC146665 |
| Gene symbol | SYNPO |
| Gene ID (NCBI) | 11346 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptopodin antibody 21064-1-AP | Download protocol |
| IHC protocol for Synaptopodin antibody 21064-1-AP | Download protocol |
| IF protocol for Synaptopodin antibody 21064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Physiol Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy. | ||
Inflammation Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model. | ||
Cell Death Dis Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy. | ||
Cell Death Dis Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress. | ||
Biochim Biophys Acta Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy. | ||
Life Sci Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy. |
