SYNPO Polyclonal antibody

Synaptopodin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21064-1-AP

Synonyms

KIAA1029, synaptopodin, SYNPO



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21064-1-AP is Synaptopodin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptopodin fusion protein Ag15089
Full Name synaptopodin
Calculated molecular weight 929 aa, 99 kDa
Observed molecular weight 99-115 kDa
GenBank accession numberBC146665
Gene symbol SYNPO
Gene ID (NCBI) 11346
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

Protocols

Product Specific Protocols
WB protocol for Synaptopodin antibody 21064-1-APDownload protocol
IHC protocol for Synaptopodin antibody 21064-1-APDownload protocol
IF protocol for Synaptopodin antibody 21064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IHC

J Cell Physiol

Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy.

Authors - Shan Song
mouseWB

Inflammation

Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.

Authors - Soulixay Senouthai
mouseWB,IF

Cell Death Dis

Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.

Authors - Kewei Xie
mouseIF

Cell Death Dis

Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress.

Authors - Yuexin Tian
humanWB

Biochim Biophys Acta

Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy.

Authors - Pan Gao
mouseIF

Life Sci

Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy.

Authors - Qiwang Ma