SYNPO Polyclonal antibody
Synaptopodin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21064-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | rat brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 16 publications below |
IHC | See 3 publications below |
IF | See 15 publications below |
Product Information
The immunogen of 21064-1-AP is Synaptopodin Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synaptopodin fusion protein Ag15089 |
Full Name | synaptopodin |
Calculated molecular weight | 929 aa, 99 kDa |
Observed molecular weight | 99-115 kDa |
GenBank accession number | BC146665 |
Gene symbol | SYNPO |
Gene ID (NCBI) | 11346 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)
Protocols
Product Specific Protocols | |
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WB protocol for Synaptopodin antibody 21064-1-AP | Download protocol |
IHC protocol for Synaptopodin antibody 21064-1-AP | Download protocol |
IF protocol for Synaptopodin antibody 21064-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Physiol Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy. | ||
Inflammation Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model. | ||
Cell Death Dis Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy. | ||
Cell Death Dis Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress. | ||
Biochim Biophys Acta Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy. | ||
Life Sci Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy. |